Human ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody

Catalog #: AF3557 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3557
AF3557-SP

All ASAH2/N-acylsphingosine Amidohydrolase-2 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ASAH2/N‑acylsphingosine Amidohydrolase‑2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ASAH2/N‑acylsphingosine Amidohydrolase-2 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ASAH2/N‑acylsphingosine Amidohydrolase-2
Thr15-Ile761
Accession # AAF86240

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ASAH2/N‑acylsphingosine Amidohydrolase-2 (Catalog # 3557-AH)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human ASAH2/N‑acylsphingosine Amidohydrolase-2 (Catalog # 3557-AH), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ASAH2/N-acylsphingosine Amidohydrolase-2

The human ASAH2 gene encodes N-acylsphingosine amidohydrolase-2, also known as neutral or non-lysosomal ceramidase. ASAH2 is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein (1). The enzyme is abundant in the brush border membranes of the intestine, but is also expressed in tissues such as kidney, brain and liver (2, 3). An N-terminally truncated form of human ASAH2 has been shown to localize to mitochondria (4). A major physiological function of ASAH2 is the metabolism of dietary sphingolipids, but the enzyme may also be involved in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate (3).

References

Tani, M. et al. (2003) J. Biol. Chem. 278:10523.
Kono, M. et al. (2006) J. Biol. Chem. 281:7324.
Mitsutake, S. et al. (2001) J. Biol. Chem. 276:26249.
El Bawab, S. et al. (2000) J. Biol. Chem. 275:21508.

Long Name

Neutral Ceramidase

Entrez Gene IDs

56624 (Human); 54447 (Mouse); 114104 (Rat)

Alternate Names

ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; ASAH2; BCDase; HNAC1; LCDase; Nacylsphingosine Amidohydrolase2; NCDase; N-CDase

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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