Human ASGR2 Antibody

Catalog #: MAB9970 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9970-100
MAB9970-SP

Detection of Human ASGR2 by Western Blot.

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All ASGR2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ASGR2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ASGR2 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2327C

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ASGR2
Gln80-Ala311
Accession # P07307

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

Immunohistochemistry

1-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ASGR2 by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for ASGR2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of ASGR2 in HEK293 Human Cell Line Transfected with Human ASGR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ASGR2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry

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ASGR2 in Human Liver. ASGR2 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: ASGR2

Asialoglycoprotein receptor 2 (ASGR2) is a subunit of the Asialoglycoprotein receptor. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the less abundant minor subunit. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. Expressed on hepatocytes, the Asialoglycoprotein receptor mediates endocytosis and lysosomal degradation of glycoproteins to mediate serum glycoprotein homeostasis. The ASGR receptor binds plasma glycoproteins which have had the terminal sialic acid residue removed. An alternatively spliced variant, H2, which can be shed, has been proposed as a marker for liver fibrosis.

Long Name

Asialoglycoprotein Receptor 2

Entrez Gene IDs

433 (Human); 11890 (Mouse); 29403 (Rat)

Alternate Names

ASGPR 2; ASGP-R 2; ASGPR2; ASGP-R2; ASGR2; asialoglycoprotein receptor 2; CLEC4H2; CLEC4H2FLJ60040; C-type lectin domain family 4 member H2; HBxAg-binding protein; HBXBP; Hepatic lectin H2; HL-2