Human ATAD2 Antibody

Catalog #: MAB9169 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9169-100
MAB9169-SP

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Human ATAD2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ATAD2 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 952947

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human ATAD2 synthetic peptide
Accession # Q6PL18

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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ATAD2 in MCF‑7 Human Cell Line. ATAD2 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human ATAD2 Monoclonal Antibody (Catalog # MAB9169) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATAD2

ATAD2/ANCCA/PRO2000 is an AAA (ATPases Associated with diverse cellular Activities) protein with two AAA domains and a bromodomain. ATAD2 is a coactivator in the regulatory process of ER alpha induction of target genes in breast cancer. Upon activation by estrogen, ATAD2 associates with ER alpha and ACTR and subsequently promotes expression of ER alpha target genes cyclin D1, c-myc and E2F1 in breast cancer cells. ATAD2 also binds and hydrolyzes ATP, mediating hypermethylation of protein complexes. ATAD2 is reported to be a prognostic biomarker in endometrial, lung, ovarian and gastric cancers. The function of ATAD2 has also been reported in other carcinomas, including cervical, hepatocellular, colorectal and lung cancers.

Long Name

ATPase family, AAA domain containing 2

Entrez Gene IDs

29028 (Human); 70472 (Mouse); 314993 (Rat)

Alternate Names

AAA nuclear coregulator cancer-associated protein; ANCCA; ANCCAMGC131938; ATAD2; ATPase family AAA domain-containing protein 2; ATPase family, AAA domain containing 2; CT137; DKFZp667N1320; EC 3.6.1.3; MGC142216; MGC29843; MGC5254; PRO2000