Human Autoimmune Regulator/AIRE PE-conjugated Antibody

Name: Human Autoimmune Regulator/AIRE PE-conjugated Antibody
Brand: R&D Systems
SKU: IC5936P

Catalog #: IC5936P Datasheet / COA / SDS

Discontinued Product

IC5936P has been discontinued.

View all Autoimmune Regulator/AIRE products.

Detection of Autoimmune Regulator/AIRE in Human Blood Monocytes by Flow Cytometry.

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Human Autoimmune Regulator/AIRE PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Autoimmune Regulator/AIRE in direct ELISAs. In Western blots, no cross-reactivity with recombinant mouse AIRE is observed.

Source

Monoclonal Mouse IgG₁ Clone # 614530

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Autoimmune Regulator/AIRE
Ser476-Ser545
Accession # O43918

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Autoimmune Regulator/AIRE in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Autoimmune Regulator/AIRE PE-conjugated Monoclonal Antibody (Catalog # IC5936P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Autoimmune Regulator/AIRE

AutoImmune REgulator (AIRE) is an approximately 60 kDa nuclear and cytosolic protein that is required for the development of T cell tolerance. It regulates the expression of self-antigens by thymic epithelial cells, and mutations in AIRE are causative of the autoimmune disorder, APECED. AIRE regulates gene transcription through interactions with DNA, histone H3, and the nuclear matrix. It contains one HSD domain (aa 1-105), a nuclear localization sequence (aa 113-133), one SAND domain (aa 181-280), and two PHD zinc finger domains (aa 299-340 and aa 434-475). Alternate splicing of human AIRE generates isoforms that lack the HSR and SAND domains and/or the second PHD domain. Within aa 476-545, human AIRE shares 65% and 63% aa sequence identity with mouse and rat AIRE, respectively.

Entrez Gene IDs

326 (Human); 11634 (Mouse); 294328 (Rat)

Alternate Names

AIRE; APECED protein; APECED; APECEDAPSI; APS1; APSI; Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein; autoimmune regulator (APECED protein)10APS1AIRE1; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy); Autoimmune Regulator; PGA1