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Human B7-1/CD80 Antibody

Catalog #: AF140
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF140
AF140-SP
Detection of Human B7‑1/CD80 by Western Blot.
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Product Details
Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human B7-1/CD80 Antibody Summary

Species Reactivity
Human
Specificity
Detects human B7-1/CD80 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human B7-2 and recombinant mouse B7-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-1/CD80
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human B7-1/CD80 antibody by Western Blot. View Larger

Detection of Human B7‑1/CD80 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF140) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for B7-1/CD80 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry B7-1/CD80 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

B7‑1/CD80 in Human Tonsil. B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human B7-1/CD80 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF140) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal center. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-1/CD80

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the
down‑regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up‑regulated through IFN-gamma. B7-1 and B7-2 are both members of the Immunoglobulin superfamily. Human B7-1 is a 288 amino acid (aa) protein containing a 34 aa signal peptide, a 208 aa extracellular domain, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Human B7-1 and B7-2 share 26% amino acid identity. Human and mouse B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form theB7-1/B7-2/CD28/CTLA-4 critical binding sites.

References
  1. Azuma, M. et al. (1993) Nature 366:76.
  2. Freeman, G.J. et al. (1993) Science 262:909.
  3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
  5. Chen, C. et al. (1994) J. Immunol. 152:4929.
  6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Entrez Gene IDs
941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

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Citations for Human B7-1/CD80 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. B7–1 Blockade Does Not Improve Post–Transplant Nephrotic Syndrome Caused by Recurrent FSGS
    Authors: Marianne Delville, Emilie Baye, Antoine Durrbach, Vincent Audard, Tomek Kofman, Laura Braun et al.
    Journal of the American Society of Nephrology
  2. Inhibition of T-cell activation by CTLA4-Fc is sufficient to ameliorate proteinuric kidney disease
    Authors: Herrera M, Soderberg M, Sabirsh A et al.
    Am. J. Physiol. Renal Physiol.
  3. Serum from minimal change patients in relapse increases CD80 expression in cultured podocytes.
    Authors: Ishimoto T, Cara-Fuentes G, Wang H et al.
    Pediatr Nephrol
  4. B7–1 Is Not Induced in Podocytes of Human and Experimental Diabetic Nephropathy
    Authors: Elena Gagliardini, Rubina Novelli, Daniela Corna, Carlamaria Zoja, Barbara Ruggiero, Ariela Benigni et al.
    Journal of the American Society of Nephrology
  5. Usefulness of the cytokines expression of Th1/Th2/Th17 and urinary CD80 excretion in adult-onset minimal change disease
    Authors: P Chen, Y Chen, M Jiang, Y Mo, H Ying, X Tang, J Zhang
    PeerJ, 2020-09-08;8(0):e9854.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Peripheral alveolar nitric oxide concentration reflects alveolar inflammation in autoimmune pulmonary alveolar proteinosis
    Authors: T Hirano, S Ohkouchi, N Tode, M Kobayashi, M Ono, T Satoh, Y Mitsuishi, A Watanabe, M Tabata, T Irokawa, H Ogawa, H Sugiura, T Kikuchi, K Akasaka, R Tazawa, Y Inoue, K Nakata, H Kurosawa, M Ichinose
    ERJ Open Res, 2018-03-23;4(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Tumor microenvironment contributes to Epstein-Barr virus anti-nuclear antigen-1 antibody production in nasopharyngeal carcinoma
    Authors: P Ai, Z Li, Y Jiang, C Song, L Zhang, H Hu, T Wang
    Oncol Lett, 2017-06-22;14(2):2458-2462.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Antigen presentation and MHC class II expression by human esophageal epithelial cells: role in eosinophilic esophagitis.
    Authors: Mulder DJ, Pooni A, Mak N, Hurlbut DJ, Basta S, Justinich CJ
    Am. J. Pathol., 2011-02-01;178(2):744-53.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Urinary CD80 is elevated in minimal change disease but not in focal segmental glomerulosclerosis.
    Authors: Garin EH, Mu W, Arthur JM
    Kidney Int., 2010-05-19;78(3):296-302.
    Species: Human
    Sample Types: Urine
    Applications: Western Blot
  10. HLA-B27-restricted antigen presentation by human chondrocytes to CD8+ T cells: potential contribution to local immunopathologic processes in ankylosing spondylitis.
    Authors: Kuhne M, Erben U, Schulze-Tanzil G, Kohler D, Wu P, Richter FJ, John T, Radbruch A, Sieper J, Appel H
    Arthritis Rheum., 2009-06-01;60(6):1635-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm.
    Authors: Sakthivel P, Shively V, Kakoulidou M
    J. Intern. Med., 2007-04-01;261(4):399-407.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  12. Induction of B7-1 in podocytes is associated with nephrotic syndrome.
    Authors: Reiser J, von Gersdorff G, Loos M, Oh J, Asanuma K, Giardino L, Rastaldi MP, Calvaresi N, Watanabe H, Schwarz K, Faul C, Kretzler M, Davidson A, Sugimoto H, Kalluri R, Sharpe AH, Kreidberg JA, Mundel P
    J. Clin. Invest., 2004-05-01;113(10):1390-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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