Human B7-H2 Antibody

Catalog #: MAB1651 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1651
MAB1651-SP

Cell Proliferation Induced by B7‑H2 and Neutralization by Human B7‑H2 Antibody.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human B7-H2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human B7-H2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H3, recombinant mouse (rm) B7-H2, or rmPD-L2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 136716

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human B7-H2
Asp19-Ser258
Accession # O75144

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7)

Neutralization

Measured by its ability to neutralize B7‑H2-induced proliferation in PHA-activated human T cells. Wang, S. et al. (2000) Blood 96:2808. The Neutralization Dose (ND₅₀) is typically 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera and 20 ng/mL Human CD3 epsilon Monoclonal Antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by B7‑H2 and Neutralization by Human B7‑H2 Antibody. In the presence of Human CD3e Monoclonal Antibody (20 ng/mL, Catalog # MAB100), Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Rec-ombinant Human B7-H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-H2 Monoclonal Antibody (Catalog # MAB1651). The ND₅₀ is typically 1-4 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H2

Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a 60 kDa member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily; the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20‑25% amino acid identity. Within the extracellular domain, human B7-H2 shares 49% and 54% amino acid sequence identity with mouse and rat B7-H2, respectively. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and T_h differentiation.

References

Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Ling, V. et al. (2000) J. Immunol. 164:1653.
Wang, S. et al. (2000) Blood 96:2808.
Brodie, D. et al. (2000) Curr. Biol. 10:333.
Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
Swallow, M.M. et al. (1999) Immunity 11:423.
Yoshinaga, S.K. et al. (1999) Nature 402:827.

Long Name

B7 Homolog 2

Entrez Gene IDs

23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)

Alternate Names

B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand