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Human B7-H2 Antibody

Catalog #: MAB165
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB165-SP
MAB165-100
MAB165-500

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Detection of B7‑H2 in U937 Human Cell Line by Flow Cytometry.
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Citations (4)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human B7-H2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human B7-H2 in direct ELISAs. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H3, or recombinant mouse B7-H2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 136726
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human B7-H2
Asp19-Ser258
Accession # O75144
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize B7‑H2-induced proliferation in PHA-activated human T cells. Wang, S. et al. (2000) Blood 96:2808. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera and 20 ng/mL Human CD3 epsilon Monoclonal Antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of B7‑H2 antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of B7‑H2 in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human B7‑H2 Monoclonal Antibody (Catalog # MAB165, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Neutralization Cell Proliferation Induced by B7‑H2 and Neutralization by Human B7‑H2 Antibody. View Larger

Cell Proliferation Induced by B7‑H2 and Neutralization by Human B7‑H2 Antibody. In the presence of Human CD3e Monoclonal Antibody (20 ng/mL, Catalog # MAB100), Recombinant Human B7-H2 Fc Chimera (Catalog # 165-B7) stimulates proliferation in PHA-activated human T cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human B7-H2 Fc Chimera (3 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human B7-H2 Monoclonal Antibody (Catalog # MAB165). The ND50 is typically 1-4 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H2

Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a 60 kDa member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20‑25% amino acid identity. Within the extracellular domain, human B7-H2 shares 49% and 54% amino acid sequence identity with mouse and rat B7-H2, respectively. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 amino acid (aa) protein with a putative 18 aa signal peptide, a 239 aa extracellular domain, an 18 aa transmembrane region, and a 33 aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

References
  1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  2. Ling, V. et al. (2000) J. Immunol. 164:1653.
  3. Wang, S. et al. (2000) Blood 96:2808.
  4. Brodie, D. et al. (2000) Curr. Biol. 10:333.
  5. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
  6. Swallow, M.M. et al. (1999) Immunity 11:423.
  7. Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
B7 Homolog 2
Entrez Gene IDs
23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)
Alternate Names
B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand

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Citations for Human B7-H2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Co-culture of primary human T cells with leukemia cells to measure regulatory T cell expansion
    Authors: Marius Külp, Laura Diehl, Halvard Bonig, Rolf Marschalek
    STAR Protocols
  2. The immune checkpoint ICOSLG is a relapse-predicting biomarker and therapeutic target in infant t(4;11) acute lymphoblastic leukemia
    Authors: Marius Külp, Anna Lena Siemund, Patrizia Larghero, Alissa Dietz, Julia Alten, Gunnar Cario et al.
    iScience
  3. ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation.
    Blood, 2012-05-10;119(26):6268-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. ICOS ligation recruits the p50alpha PI3K regulatory subunit to the immunological synapse.
    Authors: Fos C, Salles A, Lang V, Carrette F, Audebert S, Pastor S, Ghiotto M, Olive D, Bismuth G, Nunes JA
    J. Immunol., 2008-08-01;181(3):1969-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Human B7-H2 Antibody
By Geeorge Wang on 03/22/2018
Application: Flow Sample Tested: cell lines and PBMC Species: Human and Mouse