Human B7-H6 Antibody Summary
Met1-Ser262
Accession # Q68D85
Applications
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human B7‑H6 Monoclonal Antibody (Catalog # MAB71442).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human B7-H6 DuoSet ELISA (Catalog # DY7144-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Human B7‑H6 ELISA Standard Curve. Recombinant Human B7-H6 protein was serially diluted 2-fold and captured by Mouse Anti-Human B7-H6 Monoclonal Antibody (Catalog # MAB7144) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human B7-H6 Monoclonal Antibody (MAB71442) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
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B7-H6 Specificity is Shown by Flow Cytometry in Knockout Cell Line. B7-H6 knockout HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human B7-H6 Monoclonal Antibody (Catalog # MAB7144, filled histogram) or isotype control antibody (MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B). No staining in the B7-H6 knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.
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Detection of B7‑H6 in K562 cells by Flow Cytometry. K562 cells were stained with Mouse Anti-Human B7‑H6 Monoclonal Antibody (Catalog # MAB7144, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
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Detection of Human B7-H6 by Flow Cytometry Validation of cell surface proteins regulated by the US12 family.Flow cytometry confirmed proteomic data for proteins representative of each category enriched in the DAVID analysis. Staining in mock/US12 family member deletion mutant infections (blue line) is shown relative to the parental HCMV infection (red line). Flow cytometry was carried out for cell surface expression of MHC I (W6/32) as a control for HCMC infection and isotype antibody staining controls (with directly PE-conjugated IgG1, IgG2a or IgG2b antibodies or for unconjugated antibodies mIgG and an anti-mouse-AF647 conjugated secondary antibody). Infected cells were assessed by the % cells with down-regulated MHC I compared to the mock-infected cells (HCMV 94%, delta US12 94%, delta US13 94%, delta US14 83%, delta US15 95%, delta US16 97%, delta US17 85%, delta US18 94%, delta US19 96%, delta US20 94%, delta US21 94%, delta US12-21 96%). Results are representative of at least two independent experiments.DOI:https://dx.doi.org/10.7554/eLife.22206.013 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28186488), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: B7-H6
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins (1, 2). Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif (3). Both of the Ig-like domains carry N-linked glycosylation (4). Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells (4, 5). It does not show binding to NKp44, NKp46, or NKG2D (3, 6). Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis (3). B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors (3, 7). The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis (7).
- Zou, W. and L. Chen (2008) Nat. Rev. Immunol. 8:467.
- Bour-Jordan, H. et al. (2011) Immunol. Rev. 241:180.
- Brandt, C.S. et al. (2009) J. Exp. Med. 206:1495.
- Li, Y. et al. (2011) J. Exp. Med. 208:703.
- Joyce, M.G. et al. (2011) Proc. Natl. Acad. Sci. 108:6223.
- Arnon, T.I. et al. (2006) Semin. Cancer Biol. 16:348.
- Byrd, A. et al. (2007) PLoS ONE 2:e1339.
Product Datasheets
Citations for Human B7-H6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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SARS-CoV-2 host-shutoff impacts innate NK cell functions, but antibody-dependent NK activity is strongly activated through non-spike antibodies
Authors: Ceri Alan Fielding, Pragati Sabberwal, James C Williamson, Edward J D Greenwood, Thomas W M Crozier, Wioleta Zelek et al.
eLife
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Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cells
Authors: Claudia Cantoni, Martina Serra, Erica Parisi, Bruno Azzarone, Angela Rita Sementa, Luigi Aurelio Nasto et al.
OncoImmunology
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NK cell detachment from target cells is regulated by successful cytotoxicity and influences cytokine production
Authors: M Anft, P Netter, D Urlaub, I Prager, S Schaffner, C Watzl
Cell. Mol. Immunol., 2019-08-30;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Different Features of Tumor-Associated NK Cells in Patients With Low-Grade or High-Grade Peritoneal Carcinomatosis
Authors: Silvia Pesce, Valerio Belgrano, Marco Greppi, Simona Carlomagno, Margherita Squillario, Annalisa Barla et al.
Frontiers in Immunology
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B7H6 is a functional ligand for NKp30 in rat and cattle and determines NKp30 reactivity towards human cancer cell lines
Authors: EG Bjørnsen, L Thiruchelv, SE Hoelsbrekk, C Henden, PC Saether, P Boysen, MR Daws, E Dissen
Eur. J. Immunol., 2018-12-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20
Authors: Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim
Sci Rep, 2017-08-17;7(1):8661.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Control of immune ligands by members of a cytomegalovirus gene expansion suppresses natural killer cell activation
Authors: CA Fielding, MP Weekes, LV Nobre, E Ruckova, GS Wilkie, JA Paulo, C Chang, NM Suárez, JA Davies, R Antrobus, RJ Stanton, RJ Aicheler, H Nichols, B Vojtesek, J Trowsdale, AJ Davison, SP Gygi, P Tomasec, PJ Lehner, GW Wilkinson
Elife, 2017-02-10;6(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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