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Human BAK Antibody

Catalog #: AF816
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF816
AF816-SP
Detection of Human BAK by Western Blot.
3 Images
Product Details
Citations (5)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BAK Antibody Summary

Species Reactivity
Human
Specificity
Detects human BAK in Western blots. This antibody does not detect mouse BAK.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
KLH-coupled human BAK synthetic peptide
AAPADPEMVTLPLQPSSTMGC
Accession # Q16611
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1-4 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of Human Colon
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BAK antibody by Western Blot. View Larger

Detection of Human BAK by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1-4 µg/mL of Human BAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF816) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for BAK at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry BAK antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC). View Larger

BAK in HEK293 Human Cell Line. BAK was detected in immersion fixed HEK293 human embryonic kidney cell line using Rabbit Anti-Human BAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF816) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry View Larger

Detection of BAK in Human Colon. BAK was detected in immersion fixed paraffin-embedded sections of Human Colon using Rabbit Anti-Human BAK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF816) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in epithelial cells in mucosal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAK

BAK (Bcl-2 homologous antagonist/killer; also BAK1) is a 25‑30 kDa member of the BCL-2 family of proteins. It is widely expressed, and participates in the apoptotic cycle. BAK is an outer mitochondrial membrane protein that is inactive as a Zn-dependent homodimer. Upon activation by p53 or tBID, BAK oligomerizes, creating a pore in the mitochondrial membrane and allowing for cytochrome C release. Human BAK is 211 amino acids (aa) in length and contains three BCL-2 homology domains (aa 74‑88, 117‑136 and 169‑184), a Zn-binding region (aa 160‑166) and a C-terminal transmembrane segment (aa 188‑205). Amino acids 67‑94 mediate oligomerization of BAK. There are two potential isoform variants; one shows an alternate start site at Met96, while a second shows a deletion of aa 46‑66. Over amino acids 53-72, human BAK shares 55% aa identity with mouse BAK.

Long Name
Bcl-2 Antagonist/Killer
Entrez Gene IDs
578 (Human); 12018 (Mouse)
Alternate Names
Apoptosis regulator BAK; BAK; BAK1; BAK-LIKE; BAKMGC3887; BCL2-antagonist/killer 1; BCL2L7; bcl2-L-7; BCL2L7bcl-2 homologous antagonist/killer; BCL2-like 7 protein; Bcl-2-like protein 7; CDN1; MGC117255; pro-apoptotic protein BAK

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Citations for Human BAK Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. TRAF3/p38-JNK Signalling Crosstalk with Intracellular-TRAIL/Caspase-10-Induced Apoptosis Accelerates ROS-Driven Cancer Cell-Specific Death by CD40
    Authors: K Ibraheem, AMA Yhmed, MM Nasef, NT Georgopoul
    Cells, 2022-10-18;11(20):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. CD40 induces renal cell carcinoma-specific differential regulation of TRAF proteins, ASK1 activation and JNK/p38-mediated, ROS-dependent mitochondrial apoptosis
    Authors: Khalidah Ibraheem, Albashir M. A. Yhmed, Tahir Qayyum, Nicolas P. Bryan, Nikolaos T. Georgopoulos
    Cell Death Discovery
  3. A redox state-dictated signalling pathway deciphers the malignant cell specificity of CD40-mediated apoptosis
    Oncogene, 2016-11-21;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Human liver sinusoidal endothelial cells induce apoptosis in activated T cells: a role in tolerance induction.
    Authors: Karrar A, Broome U, Uzunel M, Qureshi AR, Sumitran-Holgersson S
    Gut, 2006-07-13;56(2):243-52.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Apoptotic signaling pathways induced by nitric oxide in human lymphoblastoid cells expressing wild-type or mutant p53.
    Authors: Li CQ, Robles AI, Hanigan CL, Hofseth LJ, Trudel LJ, Harris CC, Wogan GN
    Cancer Res., 2004-05-01;64(9):3022-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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