Human BANK1 Antibody

Catalog #: AF8005 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8005
AF8005-SP

Detection of Human BANK1 by Western Blot.

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Human BANK1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BANK1 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BANK1
Ser480-His785 (Cys650Arg)
Accession # Q8NDB2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BANK1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BANK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8005) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BANK1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BANK1

BANK1 (B cell scaffold protein with ANKyrin repeats) is a cytoplasmic scaffold protein containing ankyrin repeats. Although its predicted MW is 85 kDa, due to its highly acidic nature, it runs anomalously at 97-105 kDa in SDS-PAGE. It is expressed in select cell types, including mature B cells and pancreatic islet beta ‑cells. In B cells, following BCR activation, IP₃ is generated and BANK1 is phosphorylated by BCR-associated Syk (Spleen Tyr kinase). At this point, phosphorylated BANK1 subsequently interacts with both cytosolic Lyn (Lck/Yes-related Novel tyrosine kinase) and IP₃R, leading to an IP₃ receptor highly sensitive to IP₃. Increased cellular IP₃ binding to IP₃R stimulates the release of calcium from intracellular stores. BANK1 is also posited to participate in the regulation of IgM production, its role in this case being that of a negative modulator of secretion. Human BANK1 is 785 amino acids (aa) in length. It contains an IP₃R interaction region (aa 1-154), followed by a DBB domain (aa 200-327) that is involved in dimerization, and two consecutive ANK repeats (aa 345-408). There are at least three isoform variants. One utilizes an alternative start site at Met31, a second contains an eight aa substitution for aa 1-23, and a third shows a deletion of aa 24-156. BANK1 is known to form homo- and heterodimers with its isoforms, and a Arg61 to His61 transition is reported to be correlated with reduced homooligomer formation. Over aa 480-785, human BANK1 shares 73% aa sequence identity with mouse BANK1.

Long Name

B-cell Scaffold Protein with Ankyrin Repeats 1

Entrez Gene IDs

55024 (Human); 242248 (Mouse); 365948 (Rat)

Alternate Names

BANK1; BANKFLJ20706B-cell scaffold protein with ankyrin repeats; B-cell scaffold protein with ankyrin repeats 1; FLJ34204