Human BATF APC-conjugated Antibody

Catalog #: IC8054A Datasheet / COA / SDS

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IC8054A

Detection of BATF in Raji Human Cell Line by Flow Cytometry.

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All BATF Antibodies

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Human BATF APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects Human BATF in ELISA and Western Blot.

Source

Monoclonal Mouse IgG_2B Clone # 687706

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BATF
Met1-Ser125
Accession # Q16520

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of BATF antibody in Raji Human Cell Line antibody by Flow Cytometry.

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Detection of BATF in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human BATF APC-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC8054A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: BATF

BATF is a member of the AP-1 family of basic leucine zipper transcription factors. It associates with c-Jun proteins to form heterodimeric factors that inhibit transcription at AP-1 sites. The DNA binding of BATF is regulated by its phosphorylation at Ser⁴³. BATF plays a key role in several aspects of immune system development. It suppresses the differentiation of NKT and iNKT cells, while it promotes the differentiation of Th17, Th2, follicular Th cells, CD8+ T cells, and CD8+ dendritic cells. It is required for class-switch recombination in B cells and T cells as well as for germinal center formation and B cell maturation. It is also required for the expression of T cell surface proteins that mediate homing of Th cells to the gut. BATF cooperates with IRF4 in binding to composite DNA elements that are responsive to both IRF4 and AP1. Human BATF1 shares 96% amino acid sequence identity with mouse and rat BATF1

Long Name

Activating Transcription Factor 1

Entrez Gene IDs

10538 (Human); 53314 (Mouse); 299206 (Rat)

Alternate Names

basic leucine zipper transcription factor, ATF-like; BATF; BATF1basic leucine zipper transcriptional factor ATF-like; B-ATFSFA2; B-cell-activating transcription factor; SFA2; SFA-2activating transcription factor B; SF-HT-activated gene 2 protein