Human BATF3 Alexa Fluor® 488-conjugated Antibody Summary
Met1-Arg127
Accession # Q9NR55
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of BATF3 in Human PBMC Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD11c APC-conjugated Monoclonal Antibody (FAB1777A) and either (A) Mouse Anti-Human BATF3 Alexa Fluor® 488-conjugated Antigen Affinity-purified Monoclonal Antibody (Catalog # IC7437G) or (B) Mouse IgG2BAlexa Fluor 488 Isotype Control (IC0041G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
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Detection of BATF3 in Jurkat cells by Flow Cytometry Jurkat cells were stained with Mouse Anti-Human BATF3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC7437G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: BATF3
BATF3 (Basic leucine zipper transcriptional factor ATF-like 3; also p21SNFT) is a 20 kDa nuclear member of the bZIP family of proteins. It is expressed in Th1 cells and conventional dendritic cells (CD11c+), and serves to downregulate AP-1 mediated transcription. BATF3 accomplishes this by heterodimerizing with Jun and binding to AP-1 consensus binding sites, thus precluding a Jun/Fos interaction with gene activation. Human BAFT3 is 127 amino acids (aa) in length. It contains one DNA binding motif (aa 41-59) with an adjacent leucine-zipper (aa 63-84), but lacks a transactivation domain. Full-length human BATF3 (aa 1-127) shares 80% aa sequence identity with mouse BATF3.
Product Datasheets
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