Human BATF3 Antibody Summary
Met1-Arg127
Accession # Q9NR55
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of BATF3 in Human (PBMC) Monocytes by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) monocytes were stained with Mouse Anti-Human CD11c PE-conjugated Monoclonal Antibody (FAB1777P) and either (A) Mouse Anti-Human BATF3 Monoclonal Antibody (Catalog # MAB7437) or (B) Mouse IgG2BFlow Cytometry Isotype Control (MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.
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Detection of BATF3 in Jurkat cells by Flow Cytometry Jurkat cells were stained with Mouse Anti-Human BATF3 Monoclonal Antibody (Catalog # MAB7437, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BATF3
BATF3 (Basic leucine zipper transcriptional factor ATF-like 3; also p21SNFT) is a 20 kDa nuclear member of the bZIP family of proteins. It is expressed in Th1 cells and conventional dendritic cells (CD11c+), and serves to downregulate AP-1 mediated transcription. BATF3 accomplishes this by heterodimerizing with Jun and binding to AP-1 consensus binding sites, thus precluding a Jun/Fos interaction with gene activation. Human BAFT3 is 127 amino acids (aa) in length. It contains one DNA binding motif (aa 41-59) with an adjacent leucine-zipper (aa 63-84), but lacks a transactivation domain. Full-length human BATF3 (aa 1-127) shares 80% aa sequence identity with mouse BATF3.
Product Datasheets
Citation for Human BATF3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Genomic programming of IRF4-expressing human Langerhans cells
Authors: S Sirvent, AF Vallejo, J Davies, K Clayton, Z Wu, J Woo, J Riddell, VK Chaudhri, P Stumpf, LA Nazlamova, G Wheway, M Rose-Zeril, J West, M Pujato, X Chen, CH Woelk, B MacArthur, M Ardern-Jon, PS Friedmann, MT Weirauch, H Singh, ME Polak
Nat Commun, 2020-01-16;11(1):313.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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