Home / Bcl9-2 / Human Bcl9-2 Antibody

Human Bcl9-2 Antibody

Catalog #: AF4967
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4967
AF4967-SP
Detection of Human Bcl9‑2 by Western Blot.
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Bcl9-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human Bcl9-2 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Bcl9-2
Met38-Val206
Accession # Q86UU0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Bcl9‑2 antibody by Western Blot. View Larger

Detection of Human Bcl9‑2 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human Bcl9-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4967) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Bcl9-2 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Bcl9-2

Bcl9-2 (B-cell lymphoma 9-like protein; also Bcl9L and B9L) is a 200-220 kDa transcriptional regulator that belongs to the Bcl9 family of proteins. It is expressed in multiple tissues, particularly breast, and serves to recruit Pygopus to the Wnt-pathway beta -catenin-TCF complex in the nucleus. Bcl9-2 and Bcl9 are considered evolutionary duplicates of Legless that perform the same task with different regulation. Human Bcl9-2 is 1499 amino acids (aa) in length. It contains two Pro-rich regions (aa 280-493 and 891-1378). There are four potential alternate start sites at Met879, Met1000, Met708 and Met472. The last two start sites are accompanied by a deletion of aa 1241-1286, and a 62 aa substitution for the C-terminal 167 aa, respectively. Over aa 38-206, human Bcl9-2 is 94% aa identical to mouse Bcl9-2. Bcl9-2 has been identified as a phosphoprotein in several human cancer cell lines and mouse liver.

Long Name
B Cell Lymphoma/Leukemia 9-like Protein
Entrez Gene IDs
283149 (Human); 80288 (Mouse); 300673 (Rat)
Alternate Names
B-cell CLL/lymphoma 9-like protein; B-cell CLL/lymphoma 9-like; B-cell lymphoma 9-like protein; Bcl92; Bcl9-2; BCL9-like protein; DLNB11; DLNB11BCL9-2; nuclear co-factor of beta-catenin signalling; Protein BCL9-2

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human Bcl9-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Targeted Disruption of the BCL9/ beta -Catenin Complex Inhibits Oncogenic Wnt Signaling
    Authors: Kohichi Takada, Di Zhu, Gregory H. Bird, Kumar Sukhdeo, Jian-Jun Zhao, Mala Mani et al.
    Science Translational Medicine
  2. Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites
    Authors: NA Watson, TN Cartwright, C Lawless, M Cámara-Don, O Sen, K Sako, T Hirota, H Kimura, JMG Higgins
    Nat Commun, 2020-04-03;11(1):1684.
    Species: Human
    Sample Types: Cell Culture Lysates
    Applications: ELISA Capture
  3. Pharmacological inhibition of ?-catenin/BCL9 interaction overcomes resistance to immune checkpoint blockades by modulating Treg cells
    Authors: M Feng, JQ Jin, L Xia, T Xiao, S Mei, X Wang, X Huang, J Chen, M Liu, C Chen, S Rafi, AX Zhu, YX Feng, D Zhu
    Sci Adv, 2019-05-08;5(5):eaau5240.
    Species: N/A
    Sample Types: Peptides
    Applications: Immunoprecipitation
  4. Bcl9 and Pygo synergise downstream of Apc to effect intestinal neoplasia in FAP mouse models
    Authors: J Mieszczane, LM van Tienen, AEK Ibrahim, DJ Winton, M Bienz
    Nat Commun, 2019-02-13;10(1):724.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Role of BCL9L in transforming growth factor-beta (TGF-beta )-induced epithelial-to-mesenchymal-transition (EMT) and metastasis of pancreatic cancer
    Authors: Giuseppina Sannino, Nicole Armbruster, Mona Bodenhöfer, Ursula Haerle, Diana Behrens, Malte Buchholz et al.
    Oncotarget

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Bcl9-2 Antibody

There are currently no reviews for this product. Be the first to review Human Bcl9-2 Antibody and earn rewards!

Have you used Human Bcl9-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review