Home / beta-1,4-Galactosyltransferase 1/B4GalT1 / Human beta-1,4‑Galactosyltransferase 1/B4GalT1 Antibody

Human beta-1,4‑Galactosyltransferase 1/B4GalT1 Antibody

Catalog #: AF3609
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3609
AF3609-SP
Product Details
Citations (5)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human beta-1,4‑Galactosyltransferase 1/B4GalT1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta -1,4‑Galactosyltransferase 1/B4GalT1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human beta -1,4‑Galactosyltransferase 1/B4GalT1 long isoform
Gly44-Ser398
Accession # P15291
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human beta 4GalT1/ beta ‑1,4-Galactosyltransferase 1 (Catalog # 3609-GT)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human beta 4GalT1/ beta ‑1,4-Galactosyltransferase 1 (Catalog # 3609-GT), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,4-Galactosyltransferase 1/B4GalT1

beta 4GalT1 is one of seven beta 1,4 galactosyltransferases that transfer galactose in a beta 1,4 linkage to acceptor sugars including GlcNAc, and Glc, and Xyl. By sequence similarity, the beta 4GalTs form four groups: beta 4GalT1 and beta 4GalT2, beta 4GalT3 and beta 4GalT4, beta 4GalT5 and beta 4GalT6, and beta 4GalT7 (1). beta 4GalT1 is unique among the seven enzymes because it can be expressed either as membrane associated form or secreted form (2). The secreted form is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha -lactalbumin to catalyze the synthesis of lactose (3). The membrane form can reside either in the Golgi apparatus, where it adds galactose to N-acetylglucosamine residues, or on cell surface, where it functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix (4). The two enzymatic forms result from alternate transcription initiation sites and post-translational processing (5). Defects in beta 4GalT1 are the cause of congenital disorder of glycosylation type 2D (CDG2D) (6).

        

References
  1. Amado, M. et al. (1999) Biochim. Biophys. Acta. 1473:35.
  2. Yamaguchi, N and Fukuda, M.N. (1995) J. Biol. Chem. 270:12170.
  3. Appert, H.E. et al. (1986) Biochem. Biophys. Res. Commun. 138:224.
  4. Lopez, L.C. et al. (1991) J. Biol. Chem. 266:15984.
  5. Mengle-Gaw, L. et al. (1991) Biochem. Biophys. Res. Commun. 176:1269.
  6. Hansske, B. et al. (2002) J. Clin. Invest. 109:725.
Long Name
UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, Polypeptide 1
Entrez Gene IDs
2683 (Human); 14595 (Mouse); 24390 (Rat)
Alternate Names
1,4- galactosyltransferase, polypeptide 1; B4GalT1; B4GAL-T1; beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; beta4Gal-T1; betaGlcNAc beta; CDG2D; EC 2.4.1; GGTB2; GGTB2DKFZp686N19253; glycoprotein-4-beta-galactosyltransferase 2; GT1; GTB; lactose synthase; MGC50983; UDP-Gal; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

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Citations for Human beta-1,4‑Galactosyltransferase 1/B4GalT1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Shedding of N-acetylglucosaminyltransferase-V is regulated by maturity of cellular N-glycan
    Authors: Tetsuya Hirata, Misaki Takata, Yuko Tokoro, Miyako Nakano, Yasuhiko Kizuka
    Communications Biology
  2. N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion
    Authors: Laura Hobohm, Tomas Koudelka, Fenja H. Bahr, Jule Truberg, Sebastian Kapell, Sarah-Sophie Schacht et al.
    Cellular and Molecular Life Sciences
  3. Modeling Glycan Processing Reveals Golgi-Enzyme Homeostasis upon Trafficking Defects and Cellular Differentiation
    Authors: P Fisher, H Spencer, J Thomas-Oat, AJ Wood, D Ungar
    Cell Rep, 2019-04-23;27(4):1231-1243.e6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Membrane detachment is not essential for COG complex function
    Authors: LK Climer, ID Pokrovskay, JB Blackburn, VV Lupashin
    Mol. Biol. Cell, 2018-03-30;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Shedding of glycan-modifying enzymes by signal peptide peptidase-like 3 (SPPL3) regulates cellular N-glycosylation.
    Authors: Voss M, Kunzel U, Higel F, Kuhn P, Colombo A, Fukumori A, Haug-Kroper M, Klier B, Grammer G, Seidl A, Schroder B, Obst R, Steiner H, Lichtenthaler S, Haass C, Fluhrer R
    EMBO J, 2014-10-29;33(24):2890-905.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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