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Human beta-Defensin 4/2 Antibody

Catalog #: AF2758
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2758
AF2758-SP
beta -Defensin 4/2 in Human Lung.
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Citations (6)
FAQs
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human beta-Defensin 4/2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human beta -Defensin 4/2 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human beta -Defensin 4/2
Gly24-Pro64
Accession # O15263
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human beta-Defensin 4/2
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry beta -Defensin 4/2 antibody in Human Lung by Immunohistochemistry (IHC-P). View Larger

beta -Defensin 4/2 in Human Lung. beta -Defensin 4/2 was detected in immersion fixed paraffin-embedded sections of human lung using Human beta -Defensin 4/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2758) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of alveolar cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry beta -Defensin 4/2 antibody in Human Kidney Array by Immunohistochemistry (IHC-P). View Larger

beta -Defensin 4/2 in Human Kidney Array. beta -Defensin 4/2 was detected in immersion fixed paraffin-embedded sections of human kidney array using Human beta -Defensin 4/2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2758) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Defensin 2

Human BD-2, also known as defensin beta 2 (DEFB-2), is a secreted, 41 amino acid residue anti-microbial protein belonging to the defensin family. Mature defensins are cationic proteins with three intramolecular disulfides and a characteristic triple-stranded beta -sheet. Human BD-2 expression is induced by inflammatory signals in multiple tissues, including airway epithelial cells and keratinocytes. At the amino acid sequence level, human BD-2 shares 98% and 51% homology with the proteins from rhesus monkey and rat, respectively.

Long Name
Defensin 2 beta
Entrez Gene IDs
1673 (Human)
Alternate Names
BD-2; betaDefensin 2; beta-Defensin 2; DEFB2; DEFB-2; DEFB2DEFB102; DEFB4A; DEFB4B; DEFB4beta-defensin 4A; Defensin, beta 2Skin-antimicrobial peptide 1; defensin, beta 4; defensin, beta 4A; HBD-2; SAP-1; SAP1BD-2

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Citations for Human beta-Defensin 4/2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Tumor necrosis factor and norepinephrine lower the levels of human neutrophil peptides 1-3 secretion by mixed synovial tissue cultures in osteoarthritis and rheumatoid arthritis
    Authors: Birgit Riepl, Susanne Grässel, Reiner Wiest, Martin Fleck, Rainer H Straub
    Arthritis Research & Therapy
  2. Regulation of hBD-2, hBD-3, hCAP18/LL37, and Proinflammatory Cytokine Secretion by Human Milk Oligosaccharides in an Organotypic Oral Mucosal Model
    Authors: Ulvi K. Gürsoy, Krista Salli, Eva Söderling, Mervi Gürsoy, Johanna Hirvonen, Arthur C. Ouwehand
    Pathogens
  3. Gingival tissue human beta‐defensin levels in relation to infection and inflammation
    Authors: Meltem Özdemir, Feriha Caglayan, Floris J. Bikker, Pirkko Pussinen, Eija Könönen, Nermin Yamalik et al.
    Journal of Clinical Periodontology
  4. Inflammatory cytokines and antimicrobial peptides in acquired heart diseases
    Authors: E Vartina, M Pilmane, R Lacis
    Histol. Histopathol., 2019-02-08;0(0):18091.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Inflammatory and antimicrobial responses to methicillin-resistant Staphylococcus aureus in an in vitro wound infection model.
    Authors: Haisma E, Rietveld M, de Breij A, van Dissel J, El Ghalbzouri A, Nibbering P
    PLoS ONE, 2013-12-10;8(12):e82800.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. A Novel Organotypic Dento-Epithelial Culture Model: Effect of Fusobacterium nucleatum Biofilm on B-Defensin-2, -3, and LL-37 Expression.
    Authors: Gursoy UK, Pollanen M, Kononen E, Uitto VJ
    J. Periodontol., 2011-06-21;83(2):242-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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