Human beta -I Tubulin Antibody Summary
Met1-His451
Accession # Q9H4B7
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human and Mouse beta -I Tubulin by Western Blot. Western blot shows lysates of human platelets and mouse platelets. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human beta -I Tubulin Monoclonal Antibody (Catalog # MAB8527) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for beta -I Tubulin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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beta -I Tubulin in BG01V Human Embryonic Stem Cells. beta -I Tubulin was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Mouse Anti-Human beta -I Tubulin Monoclonal Antibody (Catalog # MAB8527) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of beta -I Tubulin in human tonsil. beta -I Tubulin was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human beta -I Tubulin Monoclonal Antibody (Catalog # MAB8527) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-I Tubulin
beta -tubulin is a 50 kDa cytoskeletal protein which is the a major component of neuronal processes: axons, dendrites, and dendritic spines. beta -tubulin is represented by different isoforms including beta -I, beta -II, beta -III, and beta -IV. Elimination of beta -I isoform will decrease the viability of neuroblastoma cells. Formation of actin tubules may be directly inhibited by beta -I isoform which results in decreasing cellular adhesion. beta -I Tubulin levels in different brain regions of schizophrenia patients undergo noticeable changes suggesting that this isoform may have a specific role in the pathophysiology of this illness by affecting actin tubule formation which is required for cell division and differentiation.
Product Datasheets
Citations for Human beta -I Tubulin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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WYE-354 restores Adriamycin sensitivity in multidrug-resistant acute myeloid leukemia cell lines
Authors: Sara M. Ibrahim, Sherin Bakhashab, Asad M. Ilyas, Peter N. Pushparaj, Sajjad Karim, Jalaluddin A. Khan et al.
Oncology Reports
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Susceptibility of Beavers to Chronic Wasting Disease
Authors: A Herbst, S Wohlgemuth, J Yang, AR Castle, DM Moreno, A Otero, JM Aiken, D Westaway, D McKenzie
Biology, 2022-04-26;11(5):.
Species: Transgenic Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Noncovalent reversible binding-enabled facile fabrication of leak-free PDMS microfluidic devices without plasma treatment for convenient cell loading and retrieval
Authors: B Jiang, A White, W Ou, S Van Belleg, S Stewart, JG Shamul, SO Rahaman, JP Fisher, X He
Bioactive materials, 2022-03-16;16(0):346-358.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Targeting HER3, a Catalytically Defective Receptor Tyrosine Kinase, Prevents Resistance of Lung Cancer to a Third-Generation EGFR Kinase Inhibitor
Authors: D Romaniello, I Marrocco, N Belugali N, I Ferrer, D Drago-Garc, I Vaknin, R Oren, M Lindzen, S Ghosh, M Kreitman, JC Kittel, N Gaborit, G Bergado Ba, B Sanchez, R Eilam, E Pikarsky, L Paz-Ares, Y Yarden
Cancers (Basel), 2020-08-24;12(9):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Cell Culture, Western Blot Control -
Calpain drives pyroptotic vimentin cleavage, intermediate filament loss, and cell rupture that mediates immunostimulation
Authors: MA Davis, MR Fairgrieve, A Den Hartig, O Yakovenko, B Duvvuri, C Lood, WE Thomas, SL Fink, M Gale
Proc. Natl. Acad. Sci. U.S.A., 2019-02-22;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
FAQs
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Isotype Controls
Reconstitution Buffers
Secondary Antibodies
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