Human beta-III Spectrin Antibody

Catalog #: MAB9394 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9394-100
MAB9394-SP

Detection of Human beta-III Spectrin by Western Blot.

3 Images

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human beta-III Spectrin Antibody Summary

Species Reactivity

Human

Specificity

Detects human beta-III Spectrin in direct ELISAs and Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1287A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Synthetic peptide containing the C-terminal region of beta-III Spectrin
Accession # NP_008877.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

8-25 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human beta-III Spectrin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human beta-III Spectrin Monoclonal Antibody (Catalog # MAB9394) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for beta-III Spectrin at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

View Larger

beta-III Spectrin in Human Brain. beta-III Spectrin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human beta-III Spectrin Monoclonal Antibody (Catalog # MAB9394) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry

View Larger

beta-III Spectrin in HeLa Human Cell Line. beta-III Spectrin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human beta-III Spectrin Monoclonal Antibody (Catalog # MAB9394) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-III Spectrin

Spectrin beta-III (SPTBN2) belongs to spectrins family of proteins which are essential to mechanical support/structural membrane integrity maintenance in erythrocytes, stabilizing cell-cell contacts, and localizing ion channels as well as cell adhesion molecules within specific subdomains of plasma membranes. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Spectrin beta-III interacts with EAAT4, the glutamate transporter predominately expressed in Purkinje cells, and stabilizes it at the plasma membrane leading to glutamate clearance from the synaptic cleft, and resulting in both modulation of glutamatergic neurotransmission as well as prevention of glutamate-mediated neurotoxicity.

Long Name

Spectrin Beta, Non-Erythrocytic 2

Entrez Gene IDs

6712 (Human); 29211 (Rat)

Alternate Names

beta-III Spectrin; glutamate transporter EAAT4-associated protein 41; GTRAP41; KIAA0302; SCA5; SCAR14; Spectrin beta 3; spectrin beta chain, brain 2; Spectrin beta III; spectrin, beta, non-erythrocytic 2; Spectrin, non-erythroid beta chain 2; spinocerebellar ataxia 5; SPTBN2