Human BMP-1/PCP Antibody Summary
Ala121-Gln730
Accession # NP_001190
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human BMP‑1/PCP by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human skin tissue. PVDF Membrane was probed with 2 µg/mL of Human BMP-1/PCP Monoclonal Antibody (Catalog # MAB1927) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for BMP-1/PCP at approximately 180 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-1/PCP
Bone morphogenetic protein 1 (BMP-1), also known as procollagen C-proteinase (PCP), is a zinc protease of the astacin family (1, 2). BMP-1/PCP plays a key role in formation of extracellular matrix (ECM) by converting precursor proteins into their mature and functional forms. The precursor proteins identified as substrates for BMP-1/PCP include collagens, biglycan, laminin 5, dentin matrix protein-1, and lysyl oxidase (3). There are six alternatively spliced forms known to be derived from the BMP-1 gene, and isoform 1 consisting of residues 1 to 730 was expressed. The secreted and purified protein does not contain the signal peptide (amino acid residues 1‑22) and pro domain (residues 23‑120), but contain protease (residues 121‑321), CUB I (residues 322‑434), CUB II (residues 435‑546), EGF-like (residues 547‑588) and CUB III (residues 591‑703) domains. The pro domain is apparently cleaved by a furin-like proprotein convertase (4). The purified BMP-1/PCP is an active protease and its peptidase activity can be determined as described above. The purified BMP-1/PCP is predicted to possess procollagen C-proteinase activity because it contains the minimal domain structure required (5).
- Wozney, J.M. et al. (1988) Science 242:1528.
- Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
- Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
- Leighton, M. and K.E. Kadler (2003) J. Biol. Chem. 278:18478.
- Hartigan, N. et al. (2003) J. Biol. Chem. 278:18045.
Product Datasheets
Citations for Human BMP-1/PCP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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BMP-1 disrupts cell adhesion and enhances TGF-beta activation through cleavage of the matricellular protein thrombospondin-1
Authors: Cyril Anastasi, Patricia Rousselle, Maya Talantikite, Agnès Tessier, Caroline Cluzel, Alice Bachmann et al.
Science Signaling
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Bone morphogenetic protein 1 is expressed in porcine ovarian follicles and promotes oocyte maturation and early embryonic development
Authors: Deshun Shi
J. Vet. Med. Sci., 2016-11-25;0(0):.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC -
Identification of a potential cardiac antifibrotic mechanism of torasemide in patients with chronic heart failure.
Authors: Lopez B, Gonzalez A, Beaumont J, Querejeta R, Larman M, Diez J
J. Am. Coll. Cardiol., 2007-08-13;50(9):859-67.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Structural and functional cardiac profile after prolonged duration of mechanical unloading: potential implications for myocardial recovery
Authors: Estibaliz Castillero, Ziad A. Ali, Hirokazu Akashi, Nicholas Giangreco, Catherine Wang, Eric J. Stöhr et al.
American Journal of Physiology-Heart and Circulatory Physiology
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Upregulation of Bone Morphogenetic Protein-1/Mammalian Tolloid and Procollagen C-Proteinase Enhancer-1 in Corneal Scarring
Authors: Francois Malecaze, Dawiyat Massoudi, Pierre Fournie, Cyrielle Tricoire, Myriam Cassagne, Marilyne Malbouyres et al.
Investigative Ophthalmology & Visual Science
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