Human BMP-2/BMP-4 Antibody Summary
Gln283-Arg396
Accession # P12643
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of BMP‑2/BMP‑4 in Human Lung Cancer. BMP‑2/BMP‑4 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Goat Anti-Human BMP‑2/BMP‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF355) at 0.5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-2/BMP-4
Human BMP-2 is one of at least 15 structurally and functionally related BMPs, which are members of the transforming growth factor beta (TGF-beta ) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. However, they have since been shown to be involved in embryogenesis and morphogenesis of various tissues and organs. BMPs have also been shown to regulate the growth, differentiation, chemotaxis and apoptosis of various cell types, including mesenchymal cells, epithelial cells, hematopoietic cells and neuronal cells. Similarly to other TGF-beta family proteins, BMPs are highly conserved across animal species. At the amino acid sequence level, mature human, mouse and rat BMP-2 are 100% identical. BMP-2 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. Biologically active BMP-2 is a disulfide-linked homodimer of the carboxy-terminal 114 amino acid residues that contains the characteristic seven conserved cysteine residues involved in the formation of the cysteine knot and the single interchain disulfide bond. Cellular responses to BMP-2 have been shown to be mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. One BMP type II and two BMP type I receptors have been identified. In contrast to the TGF-beta type I receptor, which does not bind the ligand in the absence of the TGF-beta receptor type II, both BMP receptor type Is can bind BMP-2 with high-affinity in the absence of BMP receptor type II.
- Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49
- Ebendal, T. et al. (1998) J. Neurosci. Res. 51:139.
- Reddi, A.H. (1998) Nature Biotechnology 16: 247.
Product Datasheets
Citations for Human BMP-2/BMP-4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Amlodipine accelerates bone healing in a stable closed femoral fracture model in mice
Authors: MM Menger, B Merscher, C Scheuer, BJ Braun, SC Herath, MF Rollmann, D Stenger, T Später, T Pohlemann, MD Menger, T Histing
European cells & materials, 2021-05-24;41(0):592-602.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Selective endocytosis of recombinant human BMPs through cell surface heparan sulfate proteoglycans in CHO cells: BMP-2 and BMP-7
Authors: MG Kim, CL Kim, YS Kim, JW Jang, GM Lee
Scientific Reports, 2021-02-09;11(1):3378.
Species: Hamster
Sample Types: Whole Cells
Applications: ICC -
Microcalcification and BMP-2 in breast cancer: correlation with clinicopathological features and outcomes
Authors: Li Zhang, Chunfang Hao, Yansheng Wu, Yuying Zhu, Yulin Ren, Zhongsheng Tong
OncoTargets and Therapy
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Evaluation of the presence of MMP-2, TIMP-2, BMP2/4, and TGF beta 3 in the facial tissue of children with cleft lip and palate
Authors: Liene Smane, Mara Pilmane
Acta medica Lituanica
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Expression of bone morphogenetic protein 2, 4, 5, and 7 correlates with histological activity of otosclerotic foci
Authors: Péter Csomor, Balázs Liktor, Bálint Liktor, Zoltán Szekanecz, István Sziklai, Tamás Karosi
Acta Oto-Laryngologica
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Midbrain Dopamine Neurons Associated with Reward Processing Innervate the Neurogenic Subventricular Zone
Authors: Jessica B. Lennington, Sara Pope, Anna E. Goodheart, Linda Drozdowicz, Stephen B. Daniels, John D. Salamone et al.
The Journal of Neuroscience
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The effect of host tissue irradiation on large-segment allograft incorporation.
Authors: Ehrhart NP, Eurell JA, Constable PD, Gaddy D, Nicholas RW
Clin. Orthop. Relat. Res., 2005-06-01;0(435):43-51.
Species: Canine
Sample Types: Whole Tissue
Applications: IHC-P -
Disruption of LTBP-4 function reduces TGF-beta activation and enhances BMP-4 signaling in the lung
Authors: Katri Koli, Frank Wempe, Anja Sterner-Kock, Anna Kantola, Martina Komor, Wolf-K. Hofmann et al.
The Journal of Cell Biology
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HOXA13 regulates the expression of bone morphogenetic proteins 2 and 7 to control distal limb morphogenesis.
Authors: Knosp WM, Scott V, Bachinger HP, Stadler HS
Development, 2004-09-01;131(18):4581-92.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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