Human BMP-2/BMP-7 Heterodimer Antibody

Catalog #: MAB3229 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3229
MAB3229-SP

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Human BMP-2/BMP-7 Heterodimer Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMP‑2/BMP‑7 Heterodimer in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) BMP-2 is observed, and no cross-reactivity with rhBMP-4/7, -4, -5, -6, -7, or -8 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 369505

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human BMP‑2/BMP‑7 Heterodimer
Ala284-Arg396 (BMP-2), Ser293-His431 (BMP-7)
Accession # NP_001191, NP_001710

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human BMP-2/BMP-7 Heterodimer (Catalog # 3229-BM) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2/BMP-7 Heterodimer

Bone Morphgenetic Protein 7 (BMP-7), also known as osteogenic protein 1 (OP-1), and BMP-2 are members of the BMP subgroup of the TGF-beta superfamily and signal through heterodimeric complexes composed of type I and type II BMP receptors. After proteolytic removal of their propeptides, BMP-2 and BMP-7 can associate into 38 kDa disulfide-linked heterodimers. The heterodimers are significantly more potent than either homodimer in osteoblast differentiation and bone formation assays. Human and mouse BMP-2 and BMP-7 share 100% and 98% aa sequence identity, respectively.

Long Name

Bone Morphogenetic Protein 2/7

Entrez Gene IDs

650 (Human)

Alternate Names

BDA2;BMP2A;Bone morphogenetic protein 2;SSFSC;SSFSC1; BMP-2/BMP-7 Heterodimer