Human BMP-3b/GDF-10 Antibody Summary
Gln369-Arg478
Accession # Q5VSQ8
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of BMP‑3b/GDF‑10 in Human Prostate Cancer. BMP‑3b/GDF‑10 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using Goat Anti-Human BMP‑3b/GDF‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1543) at 10 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMP-3b/GDF-10
BMP-3b, also known as GDF-10, belongs to the transforming growth factor beta (TGF-beta ) superfamily which includes the TGF-beta s, bone morphogenetic proteins (BMPs), growth differentiation factors (GDFs), Activins, Inhibins, Leftys, Nodal, Mullerian inhibitory substance (MIS) and the glial cell line-derived neurotrophic factors (GDNFs) (1). TGF-beta family members are synthesized and secreted as homodimeric or heterodimeric prepropeptides that are cleaved by proprotein convertases such as furin to generate the carboxy-terminal mature dimeric protein that contains the characteristic conserved cysteine residues involved in the formation of the cysteine knot domain. Within pro-BMP-3b, three dibasic cleavage sites have been identified. Mature BMP-3B is a disulfide-linked homodimer of the C-terminal 110 amino acids. Among TGF-beta family members, BMP-3b is most closely related to BMP-3, sharing 83% and 30% amino acid sequence identity in their mature and pro regions, respectively (2, 3). BMP-3b is highly conserved across animal species, the aa sequence of mature human BMP-3b is 98% identical with that of the mouse or rat proteins (2, 3). BMP-3b is expressed in developing skeletal structures of the craniofacial region and the vertebral column, as well as in the adult trachea, aorta, and most abundantly in the cerebellum and uterus (2‑4). Interestingly, in the knock-out mice, no obvious abnormalities have been found in these tissues (4). The biological function of BMP-3b is yet unknown, however studies have implicated it in the differentiation of osteoblasts, augmenting BMP-2 activity (5, 6).
- Miyazono, K. et al. (2001) J. Cell Physiol. 187:265.
- Cunningham, N.S. et al. (1995) Growth Factors 12:99.
- Takao, M. et al. (1996) Biochem. Biophys. Res. Comm. 219:656.
- Zhao, R. et al. (1999) Dev. Biol. 212:68.
- Hino, J. et al. (1999) Biochem.Biophys. Res. Comm. 256:419.
- Kaihara, S. et al. (2003) Life Sci. 72:1683.
Product Datasheets
Citation for Human BMP-3b/GDF-10 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Bone morphogenetic protein-3b (BMP-3b) is expressed in adipocytes and inhibits adipogenesis as a unique complex.
Authors: Hino J, Miyazawa T, Miyazato M, Kangawa K
Int J Obes (Lond), 2011-06-28;36(5):725-34.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
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