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Human BMP-5 Antibody

Catalog #: AF615
4 Citations Datasheet / COA / SDS
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AF615
AF615-SP
Alkaline Phosphatase Production Induced by BMP‑5 and Neutral-ization by Human BMP‑5 Antibody.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMP-5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMP‑5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) BMP‑2, rhBMP-4, rhBMP-6, rhBMP-7, and rhBMP-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMP‑5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human BMP‑5 (Catalog # 615-BMC)
Immunohistochemistry
5-15 µg/mL
Paraffin-embedded sections of human placenta and osteosarcoma
Neutralization
Measured by its ability to neutralize BMP‑5-induced alkaline phosphatase production in the MC3T3‑E1 mouse preosteoblast cell line. Erlacher, L. et al. (1998) J. Bone Miner. Res. 13:383. The Neutralization Dose (ND50) is typically 6-24 µg/mL in the presence of 1 µg/mL Recombinant Human BMP‑5 and 50 µg/mL L-ascorbic acid.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Alkaline Phosphatase Production Induced by BMP‑5 and Neutral-ization by Human BMP‑5 Antibody. View Larger

Alkaline Phosphatase Production Induced by BMP‑5 and Neutral-ization by Human BMP‑5 Antibody. Recombinant Human BMP-5 (Catalog # 615-BMC) induces alkaline phosphatase production in the the MC3T3-E1 mouse preosteoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-5 (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF615). The ND50 is typically 6-24 µg/mL in the presence of L-ascorbic acid (50 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-5

Bone Morphogenetic Protein-5 (BMP-5) is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor  beta (TGF-beta ) superfamily (1). BMP-5 is synthesized as a 454 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 20 kDa C-terminal mature protein (2). Mature BMP-5 contains seven conserved cysteine residues involved in formation of the cysteine knot and the single interchain disulfide bond. Biologically active BMP-5 is a disulfide-linked homodimer of the C-terminal mature protein. Mature human BMP-5 shares 96% aa sequence identity with mouse and rat BMP-5. Cellular responses to BMP-5 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors (1). BMP-5 is expressed by chondrocytes in proliferating and hypertrophic zones of bone growth plates (3). It contributes to limb development by promoting proliferation and differentiation of chondrocytes as well as apoptosis of undifferentiated mesoderm (3, 4). Genetic defects in BMP-5 which cause C-terminal truncation or loss of the proteolytic cleavage site result in multiple skeletal abnormalities, including the short ear phenotype in mice (5, 6). BMP-5 is also expressed by ovarian granulosa cells where it functions as an autocrine factor to promote GC proliferation and inhibit their production of progesterone (7). In the nervous system, BMP-5 promotes dendrite outgrowth and dopaminergic neuronal differentiation (8, 9). It is upregulated in oral squamous carcinoma cells and induces the apoptosis of some myeloma cell lines (10, 11).

References
  1. Chen, D. et al. (2004) Growth Factors 22:233.
  2. Celeste, A.J. et al. (1990) Proc. Natl. Acad. Sci. 87:9843.
  3. Mailhot, G. et al. (2008) J. Cell. Physiol. 214:56.
  4. Zuzarte-Luis, V. et al. (2004) Dev. Biol. 272:39.
  5. King, J.A. et al. (1994) Dev. Biol. 166:112.
  6. Ho, A.M. et al. (2008) BMC Dev. Biol. 8:35.
  7. Pierre, A. et al. (2005) Biol. Reprod. 73:1102.
  8. Beck, H.N. et al. (2001) BMC Neurosci. 2:12.
  9. Brederlau, A. et al. (2002) Mol. Cell. Neurosci. 21:367.
  10. Jin, Y. et al. (2001) Oral Oncol. 37:225.
  11. Ro, T.B. et al. (2004) Oncogene 23:3024.
Long Name
Bone Morphogenetic Protein 5
Entrez Gene IDs
653 (Human); 12160 (Mouse)
Alternate Names
BMP5; BMP-5; bone morphogenetic protein 5; MGC34244

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Citations for Human BMP-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Expression of bone morphogenetic protein 2, 4, 5, and 7 correlates with histological activity of otosclerotic foci
    Authors: Péter Csomor, Balázs Liktor, Bálint Liktor, Zoltán Szekanecz, István Sziklai, Tamás Karosi
    Acta Oto-Laryngologica
  2. Exploring Spatial Heterogeneity of Immune Cells in Nasopharyngeal Cancer
    Authors: Aastha Sobti, Christina Sakellariou, Johan S. Nilsson, David Askmyr, Lennart Greiff, Malin Lindstedt
    Cancers (Basel)
  3. Serum Proteases Potentiate BMP-Induced Cell Cycle Re-entry of Dedifferentiating Muscle Cells during Newt Limb Regeneration
    Authors: I Wagner, H Wang, PM Weissert, WL Straube, A Shevchenko, M Gentzel, G Brito, A Tazaki, C Oliveira, T Sugiura, A Shevchenko, A Simon, DN Drechsel, EM Tanaka
    Dev. Cell, 2017-03-27;40(6):608-617.e6.
    Applications: Western Blot
  4. BMP5 expression in the adult rat brain.
    Authors: Kusakawa Y, Mikawa S, Sato K
    Neuroscience, 2014-08-07;284(0):972-87.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC

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