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Human BMPR-IA/ALK-3 Antibody

Catalog #: AF346
13 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF346
AF346-SP
Detection of Human BMPR‑IA/ALK‑3 by Western Blot.
2 Images
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMPR-IA/ALK-3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human BMPR-IA in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMPR-IA/AKL-3
Gln24-Arg152
Accession # P36894
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
PC‑3 human prostate cancer cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BMPR-IA/ALK-3 antibody by Western Blot. View Larger

Detection of Human BMPR‑IA/ALK‑3 by Western Blot. Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human BMPR-IA/ALK-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF346) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BMPR-IA/ALK-3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry BMPR-IA/ALK-3 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

BMPR-IA/ALK-3 in Human Prostate Cancer Tissue. BMPR‑IA/ALK‑3 was detected in immersion fixed paraffin-embedded sections of normal human prostate tissue (negative) and human prostate cancer tissue (positive) using Goat Anti-Human BMPR‑IA/ALK‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF346) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells and stroma. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMPR-IA/ALK-3

Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor 1A (BMPR-1A), also known as activin receptor-like kinase (ALK)-3, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta  in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IA binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IA is ubiquitously expressed during embryogenesis. In adult tissues, BMPR-IA mRNA is also widely distributed with the highest expression levels found in skeletal muscle. The extracellular domain of BMPR-IA shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IA are highly conserved and share 98% sequence identity.

References
  1. Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
  2. Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.
Long Name
Bone Morphogenetic Protein Receptor IA/Activin Receptor-like Kinase 3
Entrez Gene IDs
657 (Human); 12166 (Mouse)
Alternate Names
ACVRLK310q23del; ALK-3; ALK3EC 2.7.11.30; BMP type-1A receptor; BMPR1A; BMPR-1A; BMPRIA; BMPR-IA; bone morphogenetic protein receptor type-1A; bone morphogenetic protein receptor, type IA; CD292 antigen; CD292; EC 2.7.11; Serine/threonine-protein kinase receptor R5; SKR5; type II-like kinase 3

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Citations for Human BMPR-IA/ALK-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Bioactive Lipid O-cyclic phytosphingosine-1-phosphate Promotes Differentiation of Human Embryonic Stem Cells into Cardiomyocytes via ALK3/BMPR Signaling
    Authors: Ji-Hye Jang, Min-Seong Kim, Ainsley Mike Antao, Won-Jun Jo, Hyung-Joon Kim, Su-Jin Kim et al.
    International Journal of Molecular Sciences
  2. Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival
    Authors: Maëva Chauvin, Véronique Garambois, Sylvie Choblet, Pierre-Emmanuel Colombo, Myriam Chentouf, Laurent Gros et al.
    International Journal of Oncology
  3. A new signaling cascade linking BMP4, BMPR1A, ?Np73 and NANOG impacts on stem-like human cell properties and patient outcome
    Authors: T Voeltzel, M Flores-Vio, F Zylberszte, S Lefort, M Billandon, S Jeanpierre, S Joly, G Fossard, M Milenkov, F Mazurier, A Nehme, A Belhabri, E Paubelle, X Thomas, M Michallet, F Louache, FE Nicolini, C Caron de F, V Maguer-Sat
    Cell Death Dis, 2018-09-27;9(10):1011.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, Functional Assay
  4. Chronic myeloid leukaemia cells require the bone morphogenic protein pathway for cell cycle progression and self-renewal
    Authors: P Toofan, C Busch, H Morrison, S O'Brien, H Jørgensen, M Copland, H Wheadon
    Cell Death Dis, 2018-09-11;9(9):927.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. The BMP Pathway Participates in Human Naive CD4+ T Cell Activation and Homeostasis.
    Authors: Martinez V, Sacedon R, Hidalgo L, Valencia J, Fernandez-Sevilla L, Hernandez-Lopez C, Vicente A, Varas A
    PLoS ONE, 2015-06-25;10(6):e0131453.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, IHC
  6. BMP4 inhibits breast cancer metastasis by blocking myeloid-derived suppressor cell activity.
    Authors: Cao Y, Slaney C, Bidwell B, Parker B, Johnstone C, Rautela J, Eckhardt B, Anderson R
    Cancer Res, 2014-09-15;74(18):5091-102.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Optimal effector functions in human natural killer cells rely upon autocrine bone morphogenetic protein signaling.
    Authors: Robson N, Hidalgo L, McAlpine T, Wei H, Martinez V, Entrena A, Melen G, MacDonald A, Phythian-Adams A, Sacedon R, Maraskovsky E, Cebon J, Ramirez M, Vicente A, Varas A
    Cancer Res, 2014-07-18;74(18):5019-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Bone morphogenetic protein-9 activates Smad and ERK pathways and supports human osteoclast function and survival in vitro.
    Authors: Fong D, Bisson M, Laberge G, McManus S, Grenier G, Faucheux N, Roux S
    Cell Signal, 2013-01-08;25(4):717-28.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  9. Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells.
    Authors: Lee J, Son MJ, Woolard K, Donin NM, Li A, Cheng CH, Kotliarova S, Kotliarov Y, Walling J, Ahn S, Kim M, Totonchy M, Cusack T, Ene C, Ma H, Su Q, Zenklusen JC, Zhang W, Maric D, Fine HA
    Cancer Cell, 2008-01-01;13(1):69-80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition.
    Authors: Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R
    J. Biol. Chem., 2007-06-11;282(32):23337-47.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Bone morphogenetic protein-mediated modulation of lineage diversification during neural differentiation of embryonic stem cells.
    Authors: Gossrau G, Thiele J, Konang R, Schmandt T, Brustle O
    Stem Cells, 2007-01-11;25(4):939-49.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  12. Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach.
    Authors: Bleuming SA, Kodach LL, Garcia Leon MJ, Richel DJ, Peppelenbosch MP, Reitsma PH, Hardwick JC, van den Brink GR
    J. Pathol., 2006-06-01;209(2):190-7.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Developmental pattern of expression of BMP receptors and Smads and activation of Smad1 and Smad5 by BMP9 in mouse basal forebrain.
    Authors: Lopez-Coviella I, Mellott TM, Kovacheva VP, Berse B, Slack BE, Zemelko V, Schnitzler A, Blusztajn JK
    Brain Res., 2006-04-13;1088(1):49-56.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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Human BMPR-IA/ALK-3 Antibody
By Anonymous on 10/26/2021
Application: WB Sample Tested: A549 human lung carcinoma cell line Species: Human
Western Blot Human BMPR-IA/ALK-3 Antibody AF346