Human BMPR-IB/ALK-6 Antibody Summary
Lys14-Arg126
Accession # O00238
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human BMPR-IB/ ALK-6 Monoclonal Antibody (Catalog # MAB5051, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
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Detection of BMPR-IB/ALK-6 in Human iPS cells differentiated to Mesoderm by Flow Cytometry. Human iPS cells differentiated to mesoderm (using Catalog # SC030B) were stained with Mouse Anti-Human BMPR-IB/ALK-6 Monoclonal Antibody (Catalog # MAB5051, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibosy (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
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BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line. BMPR-IB/ALK-6 was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human BMPR-IB/ALK-6 Monoclonal Antibody (Catalog # MAB5051) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BMPR-IB/ALK-6
Cellular responses to bone morphogenetic proteins (BMPs) have been shown to be mediated by the formation of hetero-oligomeric complexes of the type I and type II serine/threonine kinase receptors. BMP receptor IB (BMPR-IB), also known as activin receptor-like kinase (ALK)-6, is one of seven known type I serine/threonine kinases that are required for the signal transduction of TGF-beta family cytokines. In contrast to the TGF-beta receptor system in which the type I receptor does not bind TGF-beta in the absence of the type II receptor, type I receptors involved in BMP signaling (including BMPR-IA, BMPR-IB/ALK-6, and ActR-I/ALK-2) can independently bind the various BMP family proteins in the absence of type II receptors. Recombinant soluble BMPR-IB binds BMP-4 with high-affinity in solution and is a potent BMP-4 antagonist in vitro. BMPR-IB is expressed in various tissues during embryogenesis. In adult tissues, BMPR-IB is only found in the brain. The extracellular domain of BMPR-IB shares little amino acid sequence identity with the other mammalian ALK type I receptor kinases, but the cysteine residues are conserved. Human and mouse BMPR-IB are highly conserved and share 98% amino acid sequence identity.
- Kawabata, M. et al. (1998) Cytokine and Growth Factor Reviews 9:49.
- Ebendal, T. et al. (1998) J. Neuroscience Research 51:139.
Product Datasheets
Citations for Human BMPR-IB/ALK-6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Growth factor independence underpins a paroxysmal, aggressive Wnt5aHigh/EphA2Low phenotype in glioblastoma stem cells, conducive to experimental combinatorial therapy
Authors: Nadia Trivieri, Alberto Visioli, Gandino Mencarelli, Maria Grazia Cariglia, Laura Marongiu, Riccardo Pracella et al.
Journal of Experimental & Clinical Cancer Research
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Single Cell Center of Mass for the Analysis of BMP Receptor Heterodimers Distributions
Authors: Hendrik Boog, Rebecca Medda, Elisabetta Ada Cavalcanti-Adam
Journal of Imaging
Species: Human
Sample Types: Whole Cells
Applications: Immunocytochemistry -
Anti-Müllerian hormone concentration regulates activin receptor-like kinase-2/3 expression levels with opposing effects on ovarian cancer cell survival
Authors: Maëva Chauvin, Véronique Garambois, Sylvie Choblet, Pierre-Emmanuel Colombo, Myriam Chentouf, Laurent Gros et al.
International Journal of Oncology
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Endocytosis contributes to BMP2-induced Smad signalling and neuronal growth
Authors: SV Hegarty, AM Sullivan, GW O'Keeffe
Neurosci. Lett, 2017-02-08;643(0):32-37.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
The combined mechanism of bone morphogenetic protein- and calcium phosphate-induced skeletal tissue formation by human periosteum derived cells.
Authors: Bolander J, Ji W, Geris L et al.
Eur Cell Mater.
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The cells were stained with 1µl of anti-BMPR1 antibody in 100 µl FACS staining buffer for 30 min at 4C. After washing, the cells were incubated with 1µl of anti-mouse IgG2b-APC antibody in 100 µl FACS staining buffer for 20 min at 4C.
