Human BRCA2 Antibody

Detection of Human BRCA2 by Western Blot. Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 0.2 µg/mL of Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for BRCA2 at approximately 380 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

BRCA2 in Human Breast Cancer Tissue. BRCA2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human BRCA2 Monoclonal Antibody (Catalog # MAB2476) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in epithelial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Human Human BRCA2 Antibody by Western Blot Radioresistant IPF fibroblasts upregulate FoxM1, RAD51, and BRCA2 following exposure to radiation.a Upper, representative images showing FoxM1, RAD51, and BRCA2 protein expression in randomly selected control (n = 8) and IPF (n = 8) fibroblasts before and after 9 Gy radiation. beta -actin was used as a control. Lower, statistical analysis of FoxM1, RAD51, and BRCA2 protein expression in control and IPF fibroblasts (n = 8, each) before and after 9 Gy radiation. b Representative images and densitometry analysis of nuclear FoxM1 in control and IPF fibroblasts (n = 8, each) at 6 h after 9 Gy radiation. Lamin A/C and GAPDH were used as an internal control for nuclear and cytosolic fraction, respectively. c Changes in RAD51 mRNA expression in control and IPF fibroblasts (n = 8, each) as a function of time after 9 Gy radiation. d Changes in BRCA2 mRNA expression in control and IPF fibroblasts (n = 8) as a function of time after 9 Gy. Values are presented in mean ± SEM of fold changes compared to unirradiated control or IPF fibroblasts set at 1 fold. *: statistical significance of each protein or mRNA expression compared to unirradiated control or IPF fibroblasts at p < 0.05. e Left, control and IPF cells were irradiated with 9 Gy, and RAD51 positive cells were measured after 6 h as described in Materials and Methods. Right, statistical analysis was conducted using 3 images per each cell of 3 control or IPF fibroblasts. Scale bar indicates 50 µm. Shown is the mean fluorescence intensity (MFI) per nucleus. Values are presented in mean ± SEM of percentages compared to irradiated control fibroblasts set at 100%. Radioresistant IPF cells (high viability after 9 Gy) and radiosensitive control fibroblasts (low viability after 9 Gy) were selected for the experiment Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29789556), licensed under a CC-BY license. Not internally tested by R&D Systems.