Human BRDG1 Antibody

Catalog #: AF5717 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5717
AF5717-SP

Detection of Human BRDG1 by Western Blot.

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Human BRDG1 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human BRDG1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BRDG1
Ala177-Ser280
Accession # Q9ULZ2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BRDG1 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human BRDG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5717) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BRDG1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BRDG1

BCR downstream-signaling protein 1 (BRDG1; also known as Signal-transducing adaptor protein 1, STAP1) is a 36‑37 kDa adaptor molecule that is involved in inflammation. It is expressed in hematopoietic cells, including B cells, macrophages and microglia. BRDG1 is believed to promote and prolong the immune response by controlling cell migration, secretion and phagocytosis. It does so by likely controlling the recruitment of signaling kinases to TRKs, and potentiating BCR-mediated activation of CREB and Elk‑1. Human BRDG1 is 295 amino acids (aa) in length and contains a plextrin homology domain that binds to membrane phospholipids (aa 25‑121), and one SH2 domain that recognizes phosphorylated Tyr residues (aa 176‑259). There is a Tec-mediated phosphorylation site at Tyr65. Over amino acids 177‑280, human BRDG1 shares 82% aa identity with mouse BRDG1.

Long Name

BCR Downstream-signaling Protein 1

Entrez Gene IDs

26228 (Human); 56792 (Mouse); 305269 (Rat)

Alternate Names

BCR downstream-signaling protein 1; BRDG1; BRDG1STAP-1BCR downstream signaling 1; Docking protein BRDG1; signal transducing adaptor family member 1; signal-transducing adaptor protein 1; signal-transducing adaptor protein-1; STAP1; Stem cell adaptor protein 1