Human BTLA Antibody

Catalog #: MAB33542 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB33542-100
MAB33542-SP

Detection of BTLA in Human PBMC by Flow Cytometry.

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Human BTLA Antibody Summary

Species Reactivity

Human

Specificity

Detects human BTLA in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2161A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line, NS0-derived human BTLA
Ile25-Ser150
Accession # AAP44003

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of BTLA in Human PBMC by Flow Cytometry. Human PBMC were stained with (A) Rabbit Anti-Human BTLA Monoclonal Antibody (Catalog # MAB33542) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by PE-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110) and Mouse anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTLA

B- and T-lymphocyte attenuator (BTLA; CD272) is a 35 kDa type I transmembrane glycoprotein in the CD28 family of T cell co-stimulatory molecules (1-3). Mature human BTLA contains a 127 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane sequence, and a 111 aa cytoplasmic domain. The two ITIM motifs and three Tyr phosphorylation sites in the cytoplasmic tail transmit inhibitory signaling (4-5). The ECD of human BTLA shares 42% and 44% aa identity with that of mouse and rat BTLA, respectively. A splice variant lacking the transmembrane domain has been reported (6). Unlike other CD28 family members, the BTLA Ig domain in the ECD is of the I-type rather than V-type, and BTLA does not form homodimers (7). BTLA is also unusual in its interaction with the TNF superfamily member HVEM rather than with B7 family ligands (8). BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and NK cells (9). Its expression is low in naïve T cells and increases during antigen-specific induction of anergy. In B cells, BTLA expression is highest in mature naïve cells (9). BTLA apparently limits T cell numbers, since its deletion results in overproduction of T cells, especially CD8⁺ memory T cells that are hyper-responsive to TCR cross-linking (10). Under the control of ROR gamma t and IL-7, BTLA regulates the homeostasis and inflammatory responses of gamma δT cells (11). The binding of BTLA and HVEM does not preclude the concurrent binding of other HVEM ligands such as LIGHT or Lymphotoxin-alpha (12).

References

Murphy, K.M. et al. (2006) Nat. Rev. Immunol. 6:671.
Croft, M. (2005) Trends. Immunol. 26:292.
Watanabe, N. et al. (2003) Nat. Immunol. 4:670.
Gavrieli, M. et al. (2003) Biochem. Biophys. Res. Commun. 312:1236.
Chemnitz, J.M. et al. (2006) J. Immunol. 176:6603.
Han, P. et al. (2004) J. Immunol. 172 :5931.
Compaan, D.M. et al. (2005) J. Biol. Chem. 280:39553.
Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
Hurchla, M.A. et al. (2005) J. Immunol. 174:3377.
Krieg, C. et al. (2007) Nat. Immunol. 8:162.
Bekiaris, V. et al. (2013) Immunity 39:1082.
Cai G and Freeman GJ, (2009) Immunol Rev. 229:244

Long Name

B- And T-Lymphocyte Attenuator

Entrez Gene IDs

151888 (Human); 208154 (Mouse); 102144877 (Cynomolgus Monkey)

Alternate Names

B and T lymphocyte associated; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA1; CD272 antigen; CD272; FLJ16065; MGC129743