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Human BTLA PerCP-conjugated Antibody

Catalog #: FAB3354C
1 Citation Datasheet / COA / SDS

Discontinued Product

FAB3354C has been discontinued.
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Detection of BTLA in Human Whole Blood Lymphocytes by Flow Cytometry.
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Human BTLA PerCP-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human BTLA in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BTLA
Ile25-Ser150
Accession # AAP44003
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of BTLA antibody in Human Whole Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of BTLA in Human Whole Blood Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Goat Anti-Human BTLA PerCP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3354C) or (B) Normal Goat IgG PerCP Control (Catalog # IC108C). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: BTLA

B and T Lymphocyte Attenuator (BTLA) also known as CD272, is a 70kDa type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one V‑type Ig-like domain, which interacts with the herpesvirus entry mediator (HVEM/TNFSF14). The amino acid sequence of the ECD of human BTLA shares only 34% identity to that of mouse BTLA. One human BTLA variant lacking the transmembrane sequence has been reported.

Long Name
B- And T-Lymphocyte Attenuator
Entrez Gene IDs
151888 (Human); 208154 (Mouse); 102144877 (Cynomolgus Monkey)
Alternate Names
B and T lymphocyte associated; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA; BTLA1; CD272 antigen; CD272; FLJ16065; MGC129743

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Citation for Human BTLA PerCP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Diagnostic value of peripheral blood immune profiling in colorectal cancer.
    Authors: Choi J, Maeng H, Lee S, Kim Y, Kim D, Lee H, Namgung J, Oh H, Kim T, Jeong J, Park S, Choi Y, Kang Y, Yoon S, Lee J
    Ann Surg Treat Res, 2018-05-29;94(6):312-321.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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