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Human BTN3A1/2/3 PE-conjugated Antibody

Catalog #: FAB7136P
1 Citation Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB7136P-025
Detection of BTN3A1/2/3 in Human Blood Lymphocytes by Flow Cytometry.
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Citations (1)
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Human BTN3A1/2/3 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 849203
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD277/BTN3A1
Met1-Gly254
Accession # O00481
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD277/BTN3A1 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of BTN3A1/2/3 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human BTN3A1/2/3 PE-conjugated Monoclonal Antibody (Catalog # FAB7136P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: BTN3A1/2/3

Butyrophilin BTN3A1, also known as CD277, has high structural homology to the B7 superfamily of proteins and is expressed in various immune cells such as T and NK cells. BTN3A1 enhances TCR-induced cytokine production and cell proliferation. Early T-cell activation events such as TCR-induced cell signaling are increased upon BTN3A1 engagement. The BTN3A1 co-stimulatory pathway may be involved in the regulation of various cell-mediated immune responses.

Long Name
Butyrophilin, Subfamily 3, Member A1/2/3
Alternate Names
BT3.1; BTF5; BTN3.1; BTN3A1/2/3; butyrophilin subfamily 3 member A1; CD277; dJ45P21.3 (butyrophilin, subfamily 3, member A1)

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Citation for Human BTN3A1/2/3 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. CRISPR screens decode cancer cell pathways that trigger ?? T cell detection
    Authors: Mamedov, MR;Vedova, S;Freimer, JW;Sahu, AD;Ramesh, A;Arce, MM;Meringa, AD;Ota, M;Chen, PA;Hanspers, K;Nguyen, VQ;Takeshima, KA;Rios, AC;Pritchard, JK;Kuball, J;Sebestyen, Z;Adams, EJ;Marson, A;
    Nature
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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