Human C-Peptide Antibody

C-Peptide in BG01V Human Embryonic Stem Cells. C-Peptide was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic beta cells using Mouse Anti-Human C-Peptide Monoclonal Antibody (Catalog # MAB14171) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

C-Peptide in Human Pancreas. C-Peptide was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human C-Peptide Monoclonal Antibody (Catalog # MAB14171) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.