Human C-Reactive Protein/CRP QuicKit ELISA Summary
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence of human CRP in this assay. No medical histories were available for the donors used in this study.| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=10) | 2546 | 176-9946 | 2835 |
| ESTA plasma (n=20) | 2443 | 178-9519 | 2719 |
| Heparin plasma (n=20) | 2401 | 171-9550 | 2627 |
Cell Culture Supernates - Human peripheral blood mononuclear cells (PBMCs) (1 x 106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were left unstimulated or stimulated with 10 μg/mL PHA for 5 days. Aliquots of the culture supernates were removed and assayed for levels of human CRP. No detectable levels were observed.
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (ng/mL) | 3.75 | 26.1 | 4.03 | 24.8 |
| Standard Deviation | 0.146 | 1.08 | 0.195 | 1.31 |
| CV% | 3.9 | 4.1 | 4.8 | 5.3 |
Recovery
The recovery of human CRP spiked to three levels in samples throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 92-102 |
Linearity
Scientific Data
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Human CRP QuicKit Spiked Recovery Competitor Comparison CRP is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. Culture media recovery is 95% compared to 84% for the top competitor. In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices.
Product Datasheets
Preparation and Storage
Background: C-Reactive Protein/CRP
C-Reactive Protein (CRP), also known as Pentraxin 1, is a non-glycosylated protein in the Pentraxin family that also includes Pentraxin 2/SAP and Pentraxin 3/TSG-14. CRP functions as a sensor and activator of the innate immune response (1). In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation (2). In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2 (3). CRP assembles non-covalently into a 110-120 kDa cyclical pentamer (4). Mature human CRP shares 71% and 64% amino acid (aa) sequence identity with mouse and rat CRP, respectively (5).
Assay Procedure
These assays utilize an anti-tag coated microplate. Standards, samples, and controls are added to plate, followed by subsequent addition of an antibody cocktail. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.
Citation for Human C-Reactive Protein/CRP QuicKit ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Alveolar macrophages from EVALI patients and e-cigarette users: a story of shifting phenotype
Authors: Warren, KJ;Beck, EM;Callahan, SJ;Helms, MN;Middleton, E;Maddock, S;Carr, JR;Harris, D;Blagev, DP;Lanspa, MJ;Brown, SM;Paine, R;
Respiratory research
Species: Human
Sample Types: BALF
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