Human C5L2/GPR77 Antibody

Catalog #: MAB10254
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10254-100
MAB10254-SP

Detection of C5L2/GPR77 in HEK293 Human Cell Line Transfected with Human C5L2/GPR77 and eGFP by Flow Cytometry.

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All C5L2/GPR77 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human C5L2/GPR77 Antibody Summary

Species Reactivity

Human

Specificity

Detects human C5L2/GPR77 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 650918

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human C5L2/GPR77
Met1-Val337
Accession # Q9P296

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of C5L2/GPR77 in HEK293 Human Cell Line Transfected with Human C5L2/GPR77 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) C5L2/GPR77 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human C5L2/GPR77 Monoclonal Antibody (Catalog # MAB10254) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C5L2/GPR77

C5a anaphylatoxin chemotactic receptor C5a2, also known as C5L2 or GPR77, is a G protein-coupled receptor that in humans is encoded by the C5AR2. C5a2 is the receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides generated during the complement cascade that participate in host defense.

Long Name

C5a Anaphylatoxin Chemotactic Receptor C5L2

Entrez Gene IDs

27202 (Human); 319430 (Mouse); 445269 (Rat)

Alternate Names

C5a anaphylatoxin chemotactic receptor C5L2; C5L2; C5L2G-protein coupled receptor 77; G protein-coupled receptor 77; G protein-coupled receptor C5L2; GPF77; GPR77

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human C5L2/GPR77 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1