Home / Cadherin-11 / Human Cadherin-11 Antibody

Human Cadherin-11 Antibody

Catalog #: AF1790
12 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1790
AF1790-SP
Detection of Human Cadherin‑11 by Western Blot.
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Product Details
Citations (12)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cadherin-11 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cadherin-11 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Cadherin-7, rhCadherin-8, rhCadherin-10, rhCadherin‑18 and rhCadherin-20 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cadherin‑11
Phe23-Thr617
Accession # AAA35622
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
PC‑3 human prostate cancer cell line stained in buffer containing Ca2+ and Mg2+
Immunohistochemistry
5-15 µg/mL
See below
Adhesion Blockade
The adhesion of MDA-MB-231 human breast cancer cells (5 x 104 cells/well) to immobilized Recombinant Human Cadherin-11 Fc Chimera (Catalog # 1790-CA, 20 µg/mL, 100 µL/well) was maximally inhibited (70-100%) by 30 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Cadherin-11 antibody by Western Blot. View Larger

Detection of Human Cadherin‑11 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cadherin-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1790) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Cadherin-11 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Cadherin-11 antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Cadherin‑11 in Human Prostate Cancer Tissue. Cadherin-11 was detected in fomralin fixed paraffin-embedded sections of human prostate cancer tissue using Goat Anti-Human Cadherin-11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1790) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized in the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cadherin-11

The cadherin superfamily comprises a large number of membrane glycoproteins with one or more cadherin repeats, which are involved in Ca2+ dependent cell-cell adhesion. The family can be subdivided into several major subgroups, including the type I and type II classical cadherins, desmosomal cadherins, protocadherins, seven transmembrane (Flamingo) cadherins, FAT-family cadherins, T-cadherin and other unclassified cadherins (1). Cadherin-11, also known as OB-cadherin, is a type II classical cadherin. Classical cadherins are type I transmembrane proteins with an N-terminal extracellular domain containing five tandem cadherin repeats and a C-terminal cytoplasmic domain with a characteristic sequence for binding to catenins. Type I cadherins (E-, N-, P-, R-, M-, and EP-cadherin) differ from type II cadherins (cadherin-5 to -12, -18 to -20 and -22) by the presence of the HAV tripeptide motif in the most N-terminal cadherin repeat (2). Classic cadherins mediate cell-cell adhesion preferentially via homotypic interactions and form adherens juctions that have beta -catenin and p120 (ctn) at the cytoplasmic side of the junction (3, 4). Homotypic cadherin interactions also transduce outside-in and inside-out cell signals. Cadherin signaling induces various cellular processes including cell motility, actin cytoskeleton reorganization, proliferation, and differentiation (3, 4). Cadherin-11 is expressed in a variety or normal tissues of mesodermal origin including areas of the kidney and brain, in normal osteoblasts, and in tumors of the stomach, kidney, colon, breast, and bone (osteosarcoma) (5, 6). It is also differentially expressed in the embryonic brain and may be important in regulating neural development. Human Cadherin-11 exhibits a unique mRNA splice site allowing for two forms of the protein to be expressed, a full-length 796 amino acid (aa) protein and a COOH terminus-truncated variant of 693 aa. The truncated variant has a unique cytoplasmic region due to a frameshift event (3). The full-length human and mouse Cadherin-11 share 97% homology at the aa sequence level.

References
  1. Angst, B.D. et al. (2001) J. Cell Sci. 113:629.
  2. Gessner, R. and R. Tauber (2000) Ann. N.Y. Acad. Sci. 915:136.
  3. Feltes, C.M. et al. (2002) Cancer Research. 62:6688.
  4. Wheelock, J.J. and K.R. Johnson (2003) Annu. Rev. Cell Dev. Biol. 19:207.
  5. Hoffmann, I. and R. Balling (1995) Dev. Biol. 169:337.
  6. Pishvaian, M.J. et. al. (1999) Cancer Research 59:947.
Entrez Gene IDs
1009 (Human); 12552 (Mouse)
Alternate Names
CAD11OSF-4; cadherin 11, type 2, OB-cadherin (osteoblast); Cadherin11; Cadherin-11; CDH11; CDHOB; OB; OB-Cadherin; Osteoblast cadherin

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Citations for Human Cadherin-11 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Cadherin-23 mediates heterotypic cell-cell adhesion between breast cancer epithelial cells and fibroblasts.
    Authors: Apostolopoulou M, Ligon L
    PLoS ONE, 2012-03-07;7(3):e33289.
  2. Tumor Necrosis Factor and Interleukin-1 beta Upregulate NRP2 Expression and Promote SARS-CoV-2 Proliferation
    Authors: Ishitoku, M;Mokuda, S;Araki, K;Watanabe, H;Kohno, H;Sugimoto, T;Yoshida, Y;Sakaguchi, T;Masumoto, J;Hirata, S;Sugiyama, E;
    Viruses
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Evidence for the presence of synovial sheaths surrounding the extensor tendons at the metacarpophalangeal joints: a microscopy study
    Authors: YJ Dakkak, BT van Dijk, FP Jansen, LJ Wisse, M Reijnierse, AHM van der He, MC DeRuiter
    Arthritis Research & Therapy, 2022-06-24;24(1):154.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. SOX21 modulates SOX2-initiated differentiation of epithelial cells in the extrapulmonary airways
    Authors: Evelien Eenjes, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Gabriela G Edel, Floor Benthem, Lisette de Kreij-de Bruin et al.
    eLife
  5. Cellular census of human fibrosis defines functionally distinct stromal cell types and states
    Authors: TB Layton, L Williams, F McCann, M Zhang, M Fritzsche, H Colin-York, M Cabrita, MTH Ng, M Feldmann, SN Sansom, D Furniss, W Xie, J Nanchahal
    Nat Commun, 2020-06-02;11(1):2768.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  6. A Functional Tissue-Engineered Synovium Model to Study Osteoarthritis Progression and Treatment
    Authors: Robert M. Stefani, Saiti S. Halder, Eben G. Estell, Andy J. Lee, Amy M. Silverstein, Evie Sobczak et al.
    Tissue Engineering Part A
  7. Cadherin-11 mRNA and protein expression in ovarian tumors of different malignancy: No evidence of oncogenic or tumor-suppressive function
    Authors: CHARLOTTE VON BÜLOW, LETICIA OLIVEIRA-FERRER, THOMAS LÖNING, FABIAN TRILLSCH, SVEN MAHNER, KARIN MILDE-LANGOSCH
    Molecular and Clinical Oncology
  8. N-cadherin-mediated cell-cell adhesion promotes cell migration in a three-dimensional matrix.
    Authors: Shih W, Yamada S
    J Cell Sci, 2012-03-30;125(0):3661-70.
    Species: Canine
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Cadherin-11 promotes the metastasis of prostate cancer cells to bone.
    Authors: Chu K, Cheng CJ, Ye X, Lee YC, Zurita AJ, Chen DT, Yu-Lee LY, Zhang S, Yeh ET, Hu MC, Logothetis CJ, Lin SH
    Mol. Cancer Res., 2008-08-01;6(8):1259-67.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Secretion and organization of a cornea-like tissue in vitro by stem cells from human corneal stroma.
    Authors: Du Y, Sundarraj N, Funderburgh ML, Harvey SA, Birk DE, Funderburgh JL
    Invest. Ophthalmol. Vis. Sci., 2007-11-01;48(11):5038-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Accelerated formation of multicellular 3-D structures by cell-to-cell cross-linking.
    Authors: De Bank PA, Hou Q, Warner RM, Wood IV, Ali BE, Macneil S, Kendall DA, Kellam B, Shakesheff KM, Buttery LD
    Biotechnol. Bioeng., 2007-08-15;97(6):1617-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. E-cadherin cell-cell adhesion in ewing tumor cells mediates suppression of anoikis through activation of the ErbB4 tyrosine kinase.
    Authors: Kang HG, Jenabi JM, Zhang J, Keshelava N, Shimada H, May WA, Ng T, Reynolds CP, Triche TJ, Sorensen PH
    Cancer Res., 2007-04-01;67(7):3094-105.
    Species: Human
    Sample Types: Whole Cells
    Applications: Adhesion Blockade

FAQs

  1. Why does the staining protocol with this Cadherin antibody use buffers containing Ca2+ and Mg2+?

    • The staining protocol with this and other Cadherin antibodies uses buffer containing Ca2+ and Mg2+ because Cadherin function is Calcium-dependent. 

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