Human Calreticulin Antibody

Detection of Human Calreticulin by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Calreticulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3898) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Calreticulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Calreticulin in HeLa Human Cell Line. Calreticulin was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Calreticulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3898) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endoplasmic reticula. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Calreticulin in Human Pancreas. Calreticulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human Calreticulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3898) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Calreticulin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Goat Anti-Human Calreticulin Affinity-Purified Polyclonal Antibody (Catalog # AF3898, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Detection of Human Calreticulin by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and human adrenal gland tissue, loaded at 0.2 mg/mL. A specific band was detected for Calreticulin at approximately 63 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Calreticulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3898) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.