Human Calreticulin PE-conjugated Antibody

Name: Human Calreticulin PE-conjugated Antibody
Brand: R&D Systems
SKU: IC3898P

Catalog #: IC3898P Datasheet / COA / SDS

Discontinued Product

IC3898P has been discontinued.

View all Calreticulin products.

Detection of Calreticulin in HeLa Human Cell Line by Flow Cytometry.

1 Image

All Calreticulin Antibodies

Product Details

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Calreticulin PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Calreticulin in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 326203

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Calreticulin
Met1-Asn180
Accession # P27797

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Calreticulin antibody in HeLa Human Cell Line antibody by Flow Cytometry.

View Larger

Detection of Calreticulin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Calreticulin PE-conjugated Monoclonal Antibody (Catalog # IC3898P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Calreticulin

Human Calreticulin is a 55‑60 kDa, 400 amino acid (aa), variably glycosylated intra- and extracellular Ca⁺⁺-binding lectin that is ubiquitously expressed. It consists of three domains: a 180 aa N-terminal globular region, a 111 aa P-, or proline rich domain, and a 109 aa C-terminus. The 180 aa N-terminus (aa 18‑197) is termed Vasostatin. It is unclear if it is ever generated naturally via proteolytic processing. The Vasostatin domain has many functions. It binds to RNA (aa 18‑27), has autocatalytic phosphorylase activity (aa 77‑197), binds to a KxFFKR motif on steroid hormone receptors, and serves as a lectin-type chaperone for ER-localized molecules. It also shows antiangiogenic activity, presumably by binding to laminin carbohydrates and blocking endothelial cell adhesion and proliferation. Human Calreticulin is 94% aa identical to mouse and rat Calreticulin.

Entrez Gene IDs

811 (Human); 12317 (Mouse); 64202 (Rat)

Alternate Names

Autoantigen Ro; CALR; Calregulin; Calreticulin; cC1qR; CRP55; CRT; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; HEL-S-99n; RO; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA; Vasostatin