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Human CaM Kinase II delta Antibody

Catalog #: MAB4176
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4176
MAB4176-SP
Detection of Human CaM Kinase IIδ by Western Blot.
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Product Details
Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CaM Kinase II delta Antibody Summary

Species Reactivity
Human
Specificity
Detects endogenous human CaM Kinase IIδ isoforms in Western blots. In Western blots, this antibody does not cross-react with recombinant human CaMKII alpha, CaMKII beta, or CaMKII gamma.
Source
Monoclonal Mouse IgG2B Clone # 438422
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CaM Kinase IIδ
Ala402-Lys478
Accession # Q13557
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CaM Kinase IId antibody by Western Blot. View Larger

Detection of Human CaM Kinase IIδ by Western Blot. Western blot shows lysates of human heart, liver and kidney tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CaM Kinase IId Monoclonal Antibody (Catalog # MAB4176) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CaM Kinase IId isoforms at approximately 50 to 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CaM Kinase II delta

Calcium/calmodulin-dependent protein kinase type II delta (CaMKIIδ) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and δ, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKIIδ expression is ubiquitous and has been identified as the predominant CaMKII isozyme in the heart, where it is implicated in the development of cardiac hypertrophy and heart failure.

Long Name
Calcium/calmodulin-dependent Protein Kinase II delta Subunit
Entrez Gene IDs
817 (Human); 108058 (Mouse); 24246 (Rat)
Alternate Names
calcium/calmodulin-dependent protein kinase (CaM kinase) II delta; calcium/calmodulin-dependent protein kinase II delta; calcium/calmodulin-dependent protein kinase type II delta chain; calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II delta subunit; CaM Kinase II delta; CaM kinase II subunit delta; CAMK2D; CAMKD; CaMK-II delta subunit; CaMK-II subunit delta; CaM-kinase II delta chain; DKFZp686G23119; DKFZp686I2288; EC 2.7.11; EC 2.7.11.17; MGC44911

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Citations for Human CaM Kinase II delta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. CNP Promotes Antiarrhythmic Effects via Phosphodiesterase 2
    Authors: Cachorro E, G�nscht M, Schubert M et al.
    Circulation research
  2. Cellular Mechanisms of the Anti-Arrhythmic Effect of Cardiac PDE2 Overexpression
    Authors: Michael Wagner, Mirna S. Sadek, Nataliya Dybkova, Fleur E. Mason, Johann Klehr, Rebecca Firneburg et al.
    International Journal of Molecular Sciences
  3. Deletion of Dicer in smooth muscle affects voiding pattern and reduces detrusor contractility and neuroeffector transmission.
    Authors: Sadegh MK, Ekman M, Rippe C, Uvelius B, Sward K, Albinsson S
    PLoS ONE, 2012-04-27;7(4):e35882.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. MicroRNAs are essential for stretch-induced vascular smooth muscle contractile differentiation via microRNA (miR)-145-dependent expression of L-type calcium channels.
    Authors: Turczynska KM, Sadegh MK, Hellstrand P, Sward K, Albinsson S
    J. Biol. Chem., 2012-04-02;287(23):19199-206.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Exploring mechanisms of increased cardiovascular disease risk with antipsychotic medications: Risperidone alters the cardiac proteomic signature in mice
    Authors: Beauchemin M, Geguchadze R, Guntur AR, et al.
    Pharmacol. Res.

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