Human Carbohydrate Sulfotransferase 2/CHST2 Antibody

Catalog #: AF5107 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5107
AF5107-SP

Detection of Human Carbohydrate Sulfotransferase 2/CHST2 by Western Blot.

1 Image

All Carbohydrate Sulfotransferase 2/CHST2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbohydrate Sulfotransferase 2/CHST2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbohydrate Sulfotransferase 2/CHST2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) CHST1, rhCHST5, and recombinant mouse CHST7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human CHST2
Tyr76-Leu530
Accession # Q9Y4C5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carbohydrate Sulfotransferase 2/CHST2 (Catalog # 5107-ST), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human Carbohydrate Sulfotransferase 2/CHST2 by Western Blot. Western blot shows lysates of MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbohydrate Sulfotransferase 2/CHST2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5107) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbohydrate Sulfotransferase 2/CHST2 at approximately 50- 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 2/CHST2

The CHST family is comprised of 14 genes in both human and mouse. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). Human CHST2, also known as N-acetylglucosamine-6-O-sulfotransferase 1 (GlcNAc6ST-1) and Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST-2), was previously shown to act on non-reducing GlcNAc residues (3). The enzyme is known to be involved in biosynthesis of L-selectin ligand sialyl 6-sulfo Lewis X (4) and therefore plays a role in lymphocyte homing (5).

References

Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
Sakaguchi, H. et al. (2000) Biochim. Biophys. Acta 1523:269.
Uchimura, K. et al. (1998) J. Biol. Chem. 273:22577.
Li, X. et al. (2001) J. Leukoc. Biol. 69:565.
Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, p.964.
MacRae, I.J. et al. (2000) Biochemistry 39:1613.
Wu, Z.L. et al. (2002) FASEB J. 16:539.

Entrez Gene IDs

9435 (Human); 54371 (Mouse)

Alternate Names

C6ST; carbohydrate (chondroitin 6/keratan) sulfotransferase 2; carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2; Carbohydrate Sulfotransferase 2; CHST2; EC 2.8.2.-; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; GlcNAc6ST-1; GN6ST; Gn6ST-1; GST2; GST-2; N-acetylglucosamine 6-O-sulfotransferase 1