Human Carbohydrate Sulfotransferase 3/CHST3 Antibody

Catalog #: MAB7450 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7450
MAB7450-SP

Carbohydrate Sulfotransferase 3/CHST3 in Human Pancreas.

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Human Carbohydrate Sulfotransferase 3/CHST3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbohydrate Sulfotransferase 3/CHST3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CHST1 or recombinant mouse CHST3 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 799011

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 3/CHST3
Glu39-Thr479
Accession # Q7LGC8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Carbohydrate Sulfotransferase 3/CHST3 in Human Pancreas. Carbohydrate Sulfotransferase 3/CHST3 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human Carbohydrate Sulfotransferase 3/CHST3 Monoclonal Antibody (Catalog # MAB7450) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of endocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbohydrate Sulfotransferase 3/CHST3

The human CHST family is comprised of 14 enzymes. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST3, also known as chondroitin 6-O-sulfotransferase, transfers sulfate to position 6 of GalNAc residues on chondroitin sulfate (3). Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Loss of CHST3 function in human results in severe chondrodysplasia (4). CHST3 can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides (5). The enzymatic activity of the recombinant human CHST3 was measured using a phosphatase-coupled sulfotransferease assay (6).

References

Hemmerich, S. and Rosen, S. (2000) Glycobiology 10:849.
Bowman, K. G. and Bertozzi, C. R. (1999) Chem. Biol. 5:447.
Uchimura, K. et al. (2002) J. Biol. Chem. 277:1443.
Thiele, H. et al. (2004) Proc. Natl. Acad. Sci. U. S. A. 101:10155.
Yusa, A. et al. (2006) J. Biol. Chem. 281: 20393.
Prather, B. et al. (2012) Anal. Biochem. 423:86.

Entrez Gene IDs

9469 (Human); 53374 (Mouse); 84468 (Rat)

Alternate Names

C6ST1; C6ST-1; C6ST1HSD; C6STEC 2.8.2.17; carbohydrate (chondroitin 6) sulfotransferase 3; Carbohydrate Sulfotransferase 3; Chondroitin 6-O-sulfotransferase 1; Chondroitin 6-sulfotransferase; CHST3; EC 2.8.2; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0; GST-0