Human Carbonic Anhydrase III/CA3 Antibody

Catalog #: MAB2185 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2185
MAB2185-SP

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Detection of Human Carbonic Anhydrase III by Western Blot.

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All Carbonic Anhydrase III/CA3 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase III/CA3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbonic Anhydrase III/CA3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CA1, 2, 4, 5a, 5b, 6, 7, 8, or 9 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 495018

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase III/CA3
Ala2-Lys260
Accession # P07451

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunoprecipitation

25 µg/mL

Cell lysates spiked with Recombinant Human Carbonic Anhydrase III (Catalog # 2185-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Carbonic Anhydrase III by Western Blot. Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Carbonic Anhydrase III Monoclonal Antibody (Catalog # MAB2185) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Carbonic Anhydrase III at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase III/CA3

Carbonic Anhydrase is a group of metalloproteases that catalyze the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). CA3 is an approximately 29 kDa cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50‑60% of adult levels at birth (2). It is present at high levels in skeletal muscle and much lower levels in other tissues, such as cardiac and smooth muscle. The amino acid sequence of human CA3 is 99%, 90% and 92% identical to that of chimpanzee, dog and mouse/rat.

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.

Entrez Gene IDs

761 (Human)

Alternate Names

CA3; CAIII; CA-III; Car3; Carbonate dehydratase III; carbonic anhydrase 3; Carbonic Anhydrase III; carbonic anhydrase III, muscle specific; EC 4.2.1.1