Home / Carbonic Anhydrase IV/CA4 / Human Carbonic Anhydrase IV/CA4 Antibody

Human Carbonic Anhydrase IV/CA4 Antibody

Catalog #: MAB2186
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2186
MAB2186-SP
Detection of Human Carbonic Anhydrase IV/CA4 by Western Blot.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase IV/CA4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than
10% cross‑reactivity with recombinant human (rh) CA1, rhCA2, and recombinant mouse CA4 is observed. Does not cross-react with rhCA3, 8, 9, 10, 12, 13, or 14.
Source
Monoclonal Mouse IgG2B Clone # 310413
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase IV/CA4
Ala19-Lys283
Accession # P22748
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human colon
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase IV (Catalog # 2186-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Carbonic Anhydrase IV/CA4 antibody by Western Blot. View Larger

Detection of Human Carbonic Anhydrase IV/CA4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Carbonic Anhydrase IV/CA4 Monoclonal Antibody (Catalog # MAB2186) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase IV/CA4

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA4 is a
GPI‑anchored membrane enzyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and of proximal renal tubules. It functions as the principal CO2 taste sensor (2). In addition, a genetic mutation (Arg 14 to Trp in the signal peptide) of CA4 was found to co‑segregate with the RP17 form of retinitis pigmentosa in two large families and was not found in 36 unaffected family members or 100 controls (3).

References
  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Chandrashekar, J. et al. (2009) Science 326:443.
  3. Rebello, G. et al. (2004) Proc. Natl. Acad. Sci. USA 101:6617.
Entrez Gene IDs
762 (Human); 12351 (Mouse)
Alternate Names
CA4; CAIV; CA-IV; Car4; Carbonate dehydratase IV; carbonic anhydrase 4; Carbonic Anhydrase IV; carbonic anhydrase IVRP17; carbonic dehydratase IV; EC 4.2.1.1; retinitis pigmentosa 17 (autosomal dominant); RP17

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Citations for Human Carbonic Anhydrase IV/CA4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Ciliary Motility Decreased by a CO2/HCO3--Free Solution in Ciliated Human Nasal Epithelial Cells Having a pH Elevated by Carbonic Anhydrase IV
    Authors: Okamoto, S;Yasuda, M;Kawaguchi, K;Yasuoka, K;Kikukawa, Y;Asano, S;Tsujii, T;Inoue, S;Amagase, K;Inui, TA;Hirano, S;Inui, T;Marunaka, Y;Nakahari, T;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Membrane-anchored carbonic anhydrase IV interacts with monocarboxylate transporters via their chaperones CD147 and GP70
    Authors: LS Forero-Qui, S Ames, HP Schneider, A Thyssen, CD Boone, JT Andring, R McKenna, JR Casey, JW Deitmer, HM Becker
    J. Biol. Chem., 2018-11-16;0(0):.
    Species: Amphibian
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Intracellular and extracellular carbonic anhydrases cooperate non-enzymatically to enhance activity of monocarboxylate transporters.
    Authors: Klier, Michael, Andes, Fabian T, Deitmer, Joachim, Becker, Holger M
    J Biol Chem, 2013-12-12;289(5):2765-75.
    Species: Xenopus
    Sample Types: Cell Lysates, Whole Cells
    Applications: IHC-P, Western Blot
  4. Transport activity of the high-affinity monocarboxylate transporter MCT2 is enhanced by extracellular carbonic anhydrase IV but not by intracellular carbonic anhydrase II.
    Authors: Klier M, Schuler C, Halestrap AP, Sly WS, Deitmer JW, Becker HM
    J. Biol. Chem., 2011-06-16;286(31):27781-91.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  5. Studies on bicarbonate transporters and carbonic anhydrase in porcine nonpigmented ciliary epithelium.
    Authors: Shahidullah M, To CH, Pelis RM, Delamere NA
    Invest. Ophthalmol. Vis. Sci., 2008-11-14;50(4):1791-800.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC

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