Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody

Catalog #: MAB5388 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5388
MAB5388-SP

Carbonic Anhydrase VA/VB (CA5A/5B) in Human Liver.

1 Image

All Carbonic Anhydrase VA/VB (CA5A/5B) Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase VA/VB (CA5A/5B) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbonic Anhydrase VA/VB (CA5A/5B) in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CA1, 4, or CA6‑14 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 487908

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase VA/VB (CA5A/5B)
Ala40-Ser305
Accession # P35218

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Carbonic Anhydrase VA (Catalog # 3049-CA)
Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA)

Immunohistochemistry

8-25 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

View Larger

Carbonic Anhydrase VA/VB (CA5A/5B) in Human Liver. Carbonic Anhydrase VA/VB (CA5A/5B) was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Carbonic Anhydrase VA/ VB (CA5A/5B) Monoclonal Antibody (Catalog # MAB5388) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase VA/VB (CA5A/5B)

Carbonic Anhydrase is a group of metalloproteases that catalyze the reversible reaction of CO2 + H2O → HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA5A and CA5B are homologous proteins that are encoded by different genes locating in different chromosomes. However, they both are mitochondrial proteins and have low Carbonic Anhydrase activity. The amino acid sequence of human CA5A is 98%, 80% and 76% identical to that of chimpanzee, dog and mouse.

Entrez Gene IDs

763 (Human); 54233 (Rat)

Alternate Names

CA5;CA5AD;Carbonic anhydrase 5A, mitochondrial;CAV;CAVA;GS1-21A4.1; Carbonic Anhydrase VA/VB