Human Carbonic Anhydrase VB/CA5B Antibody

Catalog #: MAB3176 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3176
MAB3176-SP

All Carbonic Anhydrase VB/CA5B Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase VB/CA5B Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbonic Anhydrase VB/CA5B in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CA1, 2, 3, 4, 5A, 6, 7, 8, or 9 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 491725

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase VB/CA5B
Cys34-Pro317
Accession # Q9Y2D0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Carbonic Anhydrase VB (Catalog # 3176-CA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase VB/CA5B

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Carbonic Anhydrase VB encoded by the CA5B gene is a mitochondrial protein. In comparison with another mitochondrial CA (CA5A), CA5B has different tissue distribution and chromosomal location (2, 3). The amino acid sequence of human CA5B (residues 34 to 317) is 94%, 93%, 92%, and 74% identical to that of canine, mouse, bovine/rat, and chicken.

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Fujikawa-Adachi, K. et al. (1999) J. Biol. Chem. 274:21228.
Shah, G.N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1677.

Entrez Gene IDs

11238 (Human); 302669 (Rat)

Alternate Names

CA5B; Carbonate dehydratase VB; carbonic anhydrase 5B, mitochondrial; Carbonic Anhydrase VB; carbonic anhydrase VB, mitochondrial; carbonic dehydratase; CAVB; CA-VB; EC 4.2.1.1; MGC39962