Human Carbonic Anhydrase VB/CA5B Antibody

Catalog #: AF3176 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3176
AF3176-SP

Detection of Human Carbonic Anhydrase VB/CA5B by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase VB/CA5B Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbonic Anhydrase VB/CA5B in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CA-5A, rhCA-2, and rhCA-7 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase VB/CA5B
Cys34-Pro317
Accession # Q9Y2D0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Carbonic Anhydrase VB/CA5B by Western Blot. Western blot shows lysates of human kidney (medulla) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Carbonic Anhydrase VB/CA5B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3176) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Carbonic Anhydrase VB/CA5B at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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Carbonic Anhydrase VB/CA5B in Human Kidney. Carbonic Anhydrase VB/CA5B was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Carbonic Anhydrase VB/ CA5B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3176) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase VB/CA5B

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Carbonic Anhydrase VB encoded by the CA5B gene is a mitochondrial protein. In comparison with another mitochondrial CA (CA5A), CA5B has different tissue distribution and chromosomal location (2, 3). The amino acid sequence of human CA5B (residues 34 to 317) is 94%, 93%, 92%, and 74% identical to that of canine, mouse, bovine/rat, and chicken.

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Fujikawa-Adachi, K. et al. (1999) J. Biol. Chem. 274:21228.
Shah, G.N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1677.

Entrez Gene IDs

11238 (Human); 302669 (Rat)

Alternate Names

CA5B; Carbonate dehydratase VB; carbonic anhydrase 5B, mitochondrial; Carbonic Anhydrase VB; carbonic anhydrase VB, mitochondrial; carbonic dehydratase; CAVB; CA-VB; EC 4.2.1.1; MGC39962