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Human Carbonic Anhydrase X/CA10 Antibody

Catalog #: MAB2189
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2189
MAB2189-SP

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Detection of Human Carbonic Anhydrase X/CA10 by Western Blot.
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Human Carbonic Anhydrase X/CA10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carbonic Anhydrase X/CA10 in ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 862319
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carbonic Anhydrase X/CA10
Met1-Asn300
Accession # Q9NS85
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Carbonic Anhydrase X/CA10 antibody by Western Blot. View Larger

Detection of Human Carbonic Anhydrase X/CA10 by Western Blot. Western blot shows lysates of human brain (hippocampus) tissue. PVDF membrane was probed with 1.5 µg/mL of Mouse Anti-Human Carbonic Anhydrase X/CA10 Monoclonal Antibody (Catalog # MAB2189) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Carbonic Anhydrase X/CA10 at approximately 38-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase X/CA10

CA10 (Carbonic Anhydrase-related X), also called CARP X and Cerebral protein 15, is a cytosolic 35 kDa (predicted) member of the a-carbonic anhydrase family of molecules. Although it does not possess CA enzymatic activity due to the lack of Zn and His in its (potential) catalytic site, the recombinant protein used as an immunogen does show esterase activity. CA10 is found principally in CNS neurons and respiratory epithelium. Human CA10 is 328 amino acids (aa) in length. It possesses one carbonic anhydrase domain that contains a utilized phosphorylation site at Thr299. One potential isoform is reported that shows a 22 aa substitution for aa 277-328. Human and mouse CA10 are identical in amino acid sequence.

Entrez Gene IDs
56934 (Human)
Alternate Names
CA10; Carbonic Anhydrase X; carbonic anhydrase-related protein 10; Carbonic anhydrase-related protein X; CARP X; CARPX; CA-RPX; CARPXCA-RP X; Cerebral protein 15; cerebral protein-15; HUCEP-15

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Citation for Human Carbonic Anhydrase X/CA10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Carbonic anhydrase-related protein CA10 is an evolutionarily conserved pan-neurexin ligand
    Authors: FH Sterky, JH Trotter, SJ Lee, CV Recktenwal, X Du, B Zhou, P Zhou, J Schwenk, B Fakler, TC Sdhof
    Proc. Natl. Acad. Sci. U.S.A, 2017-02-01;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation

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