Human Carbonic Anhydrase XIII/CA13 Biotinylated Antibody

Catalog #: BAF2194 Datasheet / COA / SDS

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BAF2194

All Carbonic Anhydrase XIII/CA13 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carbonic Anhydrase XIII/CA13 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carbonic Anhydrase XIII/CA13 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) CA1, rhCA2, and rhCA3 is observed and less than 5% cross-reactivity with rhCA4, rhCA8, rhCA9, rhCA10, rhCA12, and rhCA14 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Carbonic Anhydrase XIII/CA13
Ser2-His262
Accession # Q8N1Q1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Carbonic Anhydrase XIII (Catalog # 2194-CA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase XIII/CA13

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. CA13 is a cytosolic enzyme with a unique and widespread distribution pattern as compared to the other cytosolic CAs, indicating that it may play important physiological roles in several organs (2).

References

Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Lehtonen, J. et al. (2003) J. Biol. Chem. 279: 2719.

Entrez Gene IDs

377677 (Human)

Alternate Names

CA13; carbonic anhydrase 13; Carbonic Anhydrase XIII; carbonic anhydrase XIIICarbonate dehydratase XIII; CAXIII; CA-XIII; EC 4.2.1.1; FLJ37995; MGC59868