Human Carboxylesterase 1/CES1 Antibody

Catalog #: MAB4920
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4920
MAB4920-SP

Detection of Human Carboxylesterase 1/CES1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carboxylesterase 1/CES1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carboxylesterase1/CES-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse (rm) CES-3 and no cross-reactivity with rmCES-2, rmCES-5, or recombinant human CES-2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 544623

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxylesterase1/CES-1
His19-Glu563
Accession # P23141

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carboxylesterase 1/CES1 (Catalog # 4920-CE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Carboxylesterase 1/CES1 by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Carboxylesterase 1/CES1 Monoclonal Antibody (Catalog # MAB4920) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Carboxylesterase 1/CES1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human Carboxylesterase 1/CES1 by Simple Western^TM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Carboxylesterase 1/CES1 at approximately 65 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Carboxylesterase 1/CES1 Monoclonal Antibody (Catalog # MAB4920). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxylesterase 1/CES1

Carboxylesterase 1 (CES-1) is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds (1, 2). They have broad substrate specificity ranging from small molecule esters such as phenylester to long chain fatty acid esters and thioesters. They play a major role as determinants of pharmacokinetic behavior for most therapeutic agents containing an ester. By de-esterification, they can activate or inactivate the agents. They also participate in detoxification of drugs such as cocaine and heroin in serum and liver. The resulting de-esterified metabolites are secreted out in urine. They can also detoxify organophosphate and carbamate analogues used in agrochemicals or chemical nerve agents, such as malathion, sarin, tabun, and VX. In addition to the hydrolytic activity, they can perform transesterification, a reaction important for cholesterol homeostasis. Carboxylesterase deficiency may be associated with non-Hodgkin lymphoma or B-cell lymphocytic leukemia. CES-1 shares the serine hydrolase fold observed in other esterases (3). CES-1 possesses an endoplasmic reticulum retention signal (HIEL) at its C-terminus.

References

Redinbo, M.R. and P.M. Potter (2005) Drug Discovery Today 10:313.
Satoh, T. and M. Hosokawa (2006) Chem. Biol. Interactions 162:195.
Fleming, C.D. et al. (2007) Biochemistry 46:5603.

Entrez Gene IDs

1066 (Human); 12623 (Mouse); 29225 (Rat)

Alternate Names

ACAT; acyl coenzyme A:cholesterol acyltransferase; Acyl-coenzyme A:cholesterol acyltransferase; Brain carboxylesterase hBr1; carboxylesterase 1 (monocyte/macrophage serine esterase 1); Carboxylesterase 1; carboxylesterase 2 (liver); CEH; CES1; Ces-1; CES1A2; CES2EC 3.1.1.1; cholesteryl ester hydrolase; Cocaine carboxylesterase; EC 3.1.1; Egasyn; HMSE1; HMSECES1A1; human monocyte/macrophage serine esterase 1; liver carboxylesterase 1; MGC117365; Monocyte/macrophage serine esterase; PCE-1; REH; Retinyl ester hydrolase; Serine esterase 1; SES1; Ses-1; SES1TGH; TGH; Triacylglycerol hydrolase

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Citation for Human Carboxylesterase 1/CES1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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