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Human Carboxypeptidase A1/CPA1 Antibody

Catalog #: MAB2856
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2856
MAB2856-SP
Product Details
Citations (1)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carboxypeptidase A1/CPA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carboxypeptidase A1/CPA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25% cross-reactivity with recombinant mouse (rm) Carboxypeptidase A1 is observed and no cross-reactivity with recombinant human (rh) Carboxypeptidase A2, B1, E, or recombinant mouse Carboxypeptidase A4 is observed.
Source
Monoclonal Rat IgG2B Clone # 477305
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A1/CPA1
Lys17-Tyr419
Accession # P15085
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase A1/CPA1

Carboxypeptidase A1 encoded by the CPA1 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. It is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The deduced amino acid sequence of human CPA1 consists of a signal peptide (residues 1 to 16), a pro region (residue 17 to 110), and a mature chain (residues 111 to 419). The purified rhCPA1 corresponds to the pro form.

References
  1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 812, Academic Press, San Diego.
Entrez Gene IDs
1357 (Human); 109697 (Mouse)
Alternate Names
carboxypeptidase A1 (pancreatic); Carboxypeptidase A1; CPA; CPA1; EC 3.4.17; EC 3.4.17.1

Product Datasheets

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Citation for Human Carboxypeptidase A1/CPA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Novel p.G250A Mutation Associated with Chronic Pancreatitis Highlights Misfolding-Prone Region in Carboxypeptidase A1 (CPA1)
    Authors: M Sándor, FG Thiel, M Schmid, A Demcsák, NC Morales Gr, BC Németh, S Vajda, A Hoerning, M Sahin-Tóth
    International Journal of Molecular Sciences, 2022-12-07;23(24):.
    Species: Human
    Sample Types: Protein Lysate

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