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Human Carboxypeptidase A1/CPA1 Antibody

Catalog #: AF2856 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2856
AF2856-SP
Neutralization by Human CPA1 Antibody
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Human Carboxypeptidase A1/CPA1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Carboxypeptidase A1/CPA1 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant mouse CPA1 is observed and less than 10% cross-reactivity with recombinant human CPB1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A1/CPA1
Lys17-Tyr419
Accession # P15085
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Carboxypeptidase A1/CPA1 (0.1 µg/mL, Catalog # 2856-ZN) cleavage of the fluorogenic peptide substrate Ac-Phe-Thiaphe-OH (100 µM).  The Neutralization Dose (ND50) is <15 µg/mL. 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization View Larger

Neutralization by Human CPA1 Antibody Measured by its ability to neutralize Recombinant Human Carboxypeptidase A1/CPA1 (0.1 µg/mL, 2856-ZN) cleavage of the fluorogenic peptide substrate Ac-Phe-Thiaphe-OH (100 µM). The Neutralization Dose (ND50) is <15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase A1/CPA1

Carboxypeptidase A1 encoded by the CPA1 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. It is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The deduced amino acid sequence of human CPA1 consists of a signal peptide (residues 1 to 16), a pro region (residue 17 to 110), and a mature chain (residues 111 to 419). The purified rhCPA1 corresponds to the pro form which can be activated.

References
  1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 812, Academic Press, San Diego.
Entrez Gene IDs
1357 (Human); 109697 (Mouse)
Alternate Names
carboxypeptidase A1 (pancreatic); Carboxypeptidase A1; CPA; CPA1; EC 3.4.17; EC 3.4.17.1

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