Human Carboxypeptidase A2/CPA2 Antibody

Catalog #: MAB2896 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2896
MAB2896-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Carboxypeptidase A2/CPA2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Carboxypeptidase A2/CPA2 in direct ELISAs and Western blots. In Western blots, recognizes the pro form but not the activated enzyme.

Source

Monoclonal Mouse IgG_2B Clone # 384014

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A2/CPA2
Tyr17-His417
Accession # P48052

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Carboxypeptidase A2 (Catalog # 2896-ZN)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A2/
CPA2 (Catalog # 2896-ZN), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Human Carboxypeptidase A2/CPA2 (0.1 µg/mL, Catalog # 2896‑ZN) cleavage of the fluorogenic peptide substrate N-acetyl-Phe-Thiaphe-OH (0.1 mM). The Neutralization Dose (ND₅₀) is typically 1.5 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carboxypeptidase A2/CPA2

Carboxypeptidase A2 encoded by the CPA2 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic side chains including Phe, Tyr, and Trp. The deduced amino acid sequence of human CPA2 consists of a signal peptide (residues 1 to 16), a pro region (residues 17 to 112), and a mature chain (residues 113 to 417).

References

Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 821, Academic Press, San Diego.

Entrez Gene IDs

1358 (Human)

Alternate Names

carboxypeptidase A2 (pancreatic); Carboxypeptidase A2; CPA2; EC 3.4.17; EC 3.4.17.15

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

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